C H I L E A N SO C I E T Y F O R C E L L B I O L O G Y X X VI ANNU A L M E E TING O ctober, 23 – 27th , 2012 Puerto V aras SPO NSORS A N I L L O A T C-71 E I NST I T U T O M I L E N I O , C E N T R O I N T E R D ISC IP L I N A R I O D E N E U R O C I E N C I AS D E V A L PA R A ISO C E N T R O D E I N V ES T I G A C I O N ES B I O M E D I C AS, U N A B C ENTRO F ONDAP DE RE GUL A C ION DE L G ENO M A F O N D E C Y T 1095089 Y 1100137, U. D E C H I L E F O N D E C Y T D E C O O P E R A C I O N I N T E R N A C I O N A L 1120512 Y 1110310, P. U N I V E RSI D A D C A T O L I C A D E C H I L E C E N T E R F O R M O L E C U L A R ST U D I ES O F T H E C E L L (C E M C) D E PA R T M E N T O F B ASI C SC I E N C ES, F A C U L T Y O F D E N T IST R Y, U. D E C H I L E FUNDA C ION C HIL ENA PARA BIO L O G IA C E LUL AR M I L L E N N I U M I NST I T U T E O N I M M U N O L O G Y A N D I M M U N O T H E R A PY T H E B I O M E D I C A L N E U R OSC I E N C E I NST I T U T E, A M I L L E N N I U M SC I E N C E I N I T I A T I V E (MSI) I NST I T U T E T H E F O G A R T Y I N T E R N A T I O N A L R ES E A R C H C O L L A B O R A T I O N A W A R D (F I R C A)-N I H

E X H IB IT ORS A . B R I L Y C I A L T D A – A L A T H E I A M E D I C A SA - A N D ES I M PO R T L T D A A R Q U I M E D SA – B I O C A N T – B I OSO N D A SA – ST E M C E L – B D B I OSC I E N C ES B I OSC H I L E I G SA – C I E N T E C SA – G A L E N I C A SA – G E N ESYS C H I L E L T D A G E N E X PR ESS – L A B T E C – L O N C O T E C SA – T C L L T D A T E C N I G E N SA – SI A L C H I L E – SU D E L A B SA

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

C H I L E A N SO C I E T Y F O R C E L L B I O L O G Y X X VI ANNU A L M E E TING

O C T O B E R, 23-27, 2012 PU E R T O V A R AS PRO GRA M

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Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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T U ESD A Y, O C T O B E R 23, 2012 09:00 – 13:00

Registration Convention C enter Foyer

11:30 – 12:30

Technical Lectures - C albuco Room M B F Bioscience Inc. and A rquimed C uantificación C elula r por Estereología y Reconstrucción Neu ronal en 3D Jose M aldonado, PhD, M B F L atino A mérica, B rasil Technical Lectures - T ronador Room Tecnigen A ctin C ytoskeleton and Plant Stomata Juan A . Negrón-Ber ríos, Interamerican University of Puerto Rico

12:30 – 13:30

Technical Lectures - C albuco Room BioTek Inst ruments and BiosC hile I GSA C ell-based Assays in D rug Discovery for Lead G eneration and Probing D rug E ffects Peter Banks, PhD, Scientific Director, BioTek Instruments, Inc. Technical Lectures - T ronador Room Agilent and G enexpress F rom H uman to Plants: A pplications of targeted sequence capture and next-generation sequencing technologies in studies of human genetic disorders, cancer and model organisms. Y uri Morei ra, L atin A merica F ield A pplication Scientist, Agilent.

13:30 – 15:30

L unch

16:00 – 17:00

Inauguration Volcanes Room C hair: M aría Rosa Bono Plena ry Lectu re L uis Izquierdo F ernandez C hair: Juan L ar raín S k e l e t a l M u sc l e a n d D i se a se : A j o u r n e y f r o m t h e b e n c h s i d e t o t h e b e d s i d e . E n r i q u e B r a n d a n . Department of Cell and Mo lecular Bio logy, CARE, MIFAB, Catholic Universit y o f Chile. Santiago, Chile.

17:00 – 18:00

Coffee B rea k – E xhibitors Convention C enter Foyer

18:00 – 19:30

Minisymposium I “T raffic, Signals, Receptors and Synapses” C albuco Room C hair: M aria Rosa Bono and Co-Chair: F rancisca B ronfman B D N F induces a ret rograde apoptotic signaling endosome containing p75 neurotrophin receptor (p75) in sympathetic neurons (SC Gs). Escudero C A, Galleguillos C, Flores CM, Bronfman FC. Millennium Nucleus in Regenerative Biology (MINREB), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. [email protected] Sorting nexin 17-binding cluster within the cytoplasmic tail of L RP1 mediates receptor recycling through the basolateral sorting endosome. Pamela Farfán1,2, Jiyeon Lee3, Jorge Larios1,2, Pablo Sotelo1 , Guojun Bu3 and M aría-Paz M arzolo1,2 . 1Laboratorio de Tráfico Intracelular y Señalización, Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, 2MINREB; P. Universidad Católica de Chile 3Dept. Neurosci, Mayo Clinic, Jacksonville, Florida 32224, USA.

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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F rizzled receptors in p rogenitor and immatu re neurons of the adult hippocampus. Lorena V arelaNalla r 1,2, Carla Calderon Toledo1 and Nibaldo C. Inestrosa1. 1Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, 2Centro de Investigaciones Biomedicas, Facultad de Ciencias Biologicas y Facultad de Medicina, Universidad Andres Bello, Santiago, Chile. [email protected] Diapause formation and downregulation of Insulin-li ke signaling via D A F-16/F O X O delays axonal degeneration and neuronal loss. A nd rea C alixto1, Juan S. Jara1 and Felipe A. Court1,2. 1Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected] Polycomb-G roup proteins (Pc G) E zh1 and E zh2 differentially regulate PSD-95 gene exp ression in developing hippocampal neu rons. Berta H enríquez1, Fernando J Bustos1,2,3, Francisca Blanco1,2, Alvaro Becerra1, Claudio Salas1, Felipe Simón1, Martín Montecino1,2, Brigitte van Zundert1.1Biological Sciences Faculty, Andres Bello University. 2FONDAP Center for Genome Regulation. 3University of Concepcion. [email protected] W nt signaling at the vertebrate neu romuscula r junction analyzed through in vivo elect roporation. Juan Pablo H enríquez*, Sylvain Marcellini, Daniel Sandoval, Jaime Teneb, Javier Espinoza. Department of Cell Biology and CMA Bio-Bio, Universidad de Concepcion, Concepcion, Chile. *[email protected] Minisymposium II “C ell Biology of Disease” T ronador Room C hair: M auricio González and Co-C hair: Silvana Zanlungo Novel mechanisms cont ributing to hepatic steatosis and insulin resistance in the A G PA T2 deficient mouse model of congenital generalized lipodystrophy. V íctor Cortés 1,2,, Anil Agarwal3, Abhimanyu Garg3 and Jay D. Horton2,3. 1Department of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de Chile. 2Departments of Molecular Genetics and 3Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. [email protected] M aternal supraphysiological hypercholesterolemia in p regnancy leads to placental endothelial dysfunction. Leiva A, Diez de Medina C, Guzmán-Gutiérrez E, Abarzúa E, Pardo F, Sobrevia L. Cellular and Molecular Physiology Laboratory (CMPL), Division of Obstetrics and Gynecology, Faculty of Medicine, Pontificia Universidad Católica de Chile. [email protected] M itochondrial fusion dynamics in skeletal muscle of healthy and alcoholic rat. V eronica E isner 1, Guy Lenaers2, Gyorgy Hajnoczky1. (1)Thomas Jefferson University, Philadelphia, PA. (2) INSERM U583, Institut des Neurosciences de Montpellier, France. [email protected] H emeoxygenase 1 as a therapeutic target for Systemic L upus E rithematosus. Juan P. M ackern 1, Carolina Llanos2, Leandro J. Carreño1, Camila B. Schmidt1, Ignacio Anegon3, Sergio H. Jacobelli2 and Alexis M. Kalergis1,2. 1Millennium Institute on Immunology and Immunotherapy. Departamento de Genética Molecular y Microbiología. Facultad de Ciencias Biológicas. Pontificia Universidad Católica de Chile. 2Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina, Pontificia Universidad Católica. 3INSERM U1064, Nantes, France. [email protected] C1 interaction with Trypanosoma cruzi calreticulin mediates ex vivo infection of human placenta. Castillo C, Duaso J, Maldonado I, Ramírez G, Valck C, Kemmerling U* and F er rei ra A*. ICBM, Faculty of Medicine, University of Chile. [email protected] M utations in protein disulfide isomerases a re lin ked to the development of A LS. Woehlbier U., Gonzalez-Perez P., Colombo A., Andreu C., Torres M., Valenzuela V., Diaz A., Armisen A., Sagredo A., Palma K., Concha M., Brown R. and Hetz C. Biomedical Neuroscience Institute, and Center for Molecular Studies of the Cell, ICBM, Faculty of Medicine, University of Chile, Santiago Chile and University of Massachusetts, USA. 20:30

Dinner

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

22:00 – 23:00

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Plena ry Lectu re T he Biomedical Neu roscience Institute, a M illennium Science Initiative (MSI) Institute. Volcanes Room C hair: A ndrés Couve Smell and the single neu ron: T h ree-dimensional reconst ructions of olfactory neu rons offer insights into neuronal morphologic diversity and odor coding. K ristin Baldwin. Department of Cell Biology, Scripps Res Inst, La Jolla, CA, USA.

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W E D N ESD A Y , O C T O B E R 24, 2012 08:00

Poster Mounting Session I: N° 1 to N° 80 Convention C enter Foyer

09:00 – 11:00

Symposium M illennium Institute on Immunology and Immunotherapy C albuco Room C hair: A lexis K alergis “T ranslational Immunology” E ffects of a t ransforming human ret rovi rus (H T L V) on the cellula r response to D N A damage. Susan J. M ar riott, Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas. T he ala rmin Interleukin-33 receptor and its role in inflammatory responses. M arcela H ermoso, Facultad de Medicina, Universidad de Chile. Molecula r modulation of the immunological synapse to imp rove the human immune response. A lexis M . K alergis, Facultad de Ciencias Biológicas y de Medicina, P. Universidad Católica de Chile. T he hea rtb rea k of studying glucocorticoid receptors at the N I H . John A . C idlowski, Molecular Endocrinology Group, Laboratory of Signal Transduction, NIEHS, NIH, DHHS, Research Triangle Park, North Carolina, USA Symposium T he Biomedical Neu roscience Institute, a M illennium Science Initiative (MSI) Institute and T he Fogarty International Resea rch Collaboration A ward (F I R C A)-N I H T ronador Room C hair: L isette Leyton “C ell-C ell Interactions Governing C ell M igration” Regulation of Rho G T Pase activity by adhesion molecules and mechanotransduction. K eith Bur ridge, Elizabeth Lessey, Elizabeth Monaghan-Benson and Christophe Guilluy, Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, and McAllister Heart Institute, University of North Carolina, Chapel Hill, NC 27599, USA. [email protected] Signaling in astrocyte migration induced by the neuronal protein T hy-1. Kong, M., Alvarez, A. Cárdenas, A., Quest, AFG. and L eyton, L. Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula. Facultad de Medicina. Universidad de Chile. [email protected] A dhesive and tensile interactions underlying individual and collective cell migration in vivo. Germán Reig1,3, Eduardo Pulgar1,2,3, Felipe Santibañez2,3, Valeria Larenas1,3, Ricardo Figueroa1,3, Steffen Hartell,2,3, M iguel Concha 1,3. 1Laboratory of Experimental Ontogeny – LEO and 2SCIAN-Lab, ICBM, Faculty of Medicine, University of Chile; 3Biomedical Neuroscience Institute, Santiago, Chile. [email protected]

10:30 – 13:00

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

11:00 – 13:00

Poster V iewing Session I: 1-80 O dd Numbers Convention C enter Foyer Coordinators: Rebeca A ldunate – A ndrea Paula-L ima – M ario G alindo (1) Role of c-A bl in the processing of A PP and its implication in Niemann-Pick type C disease models. Y añez M J 1,2, Alvarez AR1, Zanlungo, S2. 1Departamento de Biología Celular y Molecular,

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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Facultad de Ciencias Biológicas. 2Departamento de Gastroenterología, Facultad de Medicina. Pontificia Universidad Católica de Chile. [email protected] (3) Synaptic plasticity induced by B D N F requi res R OS and RyR-mediated C a2+ signals. $ A dasme T, $ Figueroa P, $Bruna B, #Paula-Lima AC, $,§Hidalgo C. $CEMC, BNI & §ICBM, F. Medicina, #F. Odontología, Universidad de Chile. [email protected] (5) RSV infects dendritic cells and changes tyrosine phosphorylation in T cell signaling molecules. C inthia A . A lvarez1 and Alexis Kalergis1,2. 1Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina, Pontificia Universidad Católica. [email protected] (7) T he F C fraction of immunoglobulin G attenuates the secretion of I L-10 by regulatory B cells. A ravena O, Ferrier A, Pino-Lagos K, Aguillón JC, Soto L, Catalán D. IRTGroup, Programa Disciplinario de Inmunología, ICBM, and Hospital Clínico, Universidad de Chile. [email protected] (9) Identification of Moloney M urine Leu kemia V i rus (M L V) p re-integration complex (PI C) interacting host cell p roteins. G loria A r riagada 1,2 and Stephen P. Goff1. 1Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA. 2Departamento de Ciencias Biológicas, Universidad Andrés Bello, Campus Viña del Mar. [email protected] (11) G alectin-8 is pro-fib rotic in human gingival fibroblasts. Pat ricio A vila, Anibal Romero A, Mónica Cáceres M, Patricio Smith, Andrea Soza, Alfonso González. Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas1. Departamento de Inmunología Clínica y Reumatología2, y Odontología3, Facultad de Medicina. Pontificia Universidad Católica de Chile. [email protected] (13) Intracelula r localization of cereb ral dopamine neu rotrofic factor (C D N F). A nllely Fernández, Sheyla Guzmán, Yocelyn Cruz, Marysol Paz, Jorge Escobar, Pedro Zamorano*. Faculty of Health Sciences, University of Antofagasta, Chile. *[email protected] (15) M icrotubule-associated protein 1B (M A P1B) regulates the establishment of p resynaptic contacts in vitro and interacts with the p resynaptic protein Snapin. Bodaleo F elipe1, MontenegroVenegas Carolina1, Hartel Steffen2 and González-Billault Christian1. (1)Laboratory of Cellular and Neuronal Dynamics, Department of Biology, Faculty of Sciences, Universidad de Chile. (2)Laboratory for Scientific Image Analysis, Faculty of Medicine, Universidad de Chile. [email protected] (17) A computational study of methods for estimating diffusion coefficient in frap experiments. L uis B riones1, Mauricio Cerda1, Jorge Toledo1,2, Omar Ramirez1, Takeshi Asahi3, Andres Couve2, and Steffen Härtel1. 1SCIAN-Lab, 2Couve-Lab, BNI, Faculty of Medicine, 3CMM, FCFM; U-Chile. [email protected] (19) M itochondrial and respi ratory effects of copper dep rivation in erythropoietic cells. Rodrigo Bustos, Lina Ruiz, Maria Isabel Lasso, Susana Grant and Alvaro Elorza. Experimental Bioenergetics Laboratory, Universidad Andres Bello. [email protected] (21) Post-transductional cleavage of C-terminal tail of B M P receptor type I I in motor neu ron-like cells. Pila r C á rdenas1, Juan Pablo Henríquez2 and Nelson Osses1. 1Institute of Chemistry, P. Catholic University of Valparaiso and 2Department of Cell Biology and CMA Bio-Bio, University of Concepción, Chile. [email protected] (23) Small depola rization of muscle fibers induced by external A T P can activate IP 3-dependent C a 2+ signals related to muscle plasticity. C asas, M ., Llanos, P. Hidalgo J., Jorquera, G., Buvinic S. and Jaimovich, E. Centro de Estudios Moleculares de la Célula. ICBM, Facultad de Medicina, Universidad de Chile. [email protected]

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(25) Increased angiogenesis in vitro induced by V E G F-A secreted from ca rcinoma-associated stroma of invasive prostate cancer. Javier C erda-Infante1, Ignacio San Francisco2, Alejandro Godoy3, Gary Smith3, Viviana Montecinos1. 1Hematology-Oncology and 2Urology Departments, Pontificia Universidad Católica de Chile. 3Urology department, Roswell Park Cancer Institute, Buffalo NY. [email protected] (Sponsor: M.A. García). (27) T r kB receptor induces the activation and nuclea r translocation of the ty rosine kinase c- A bl in hippocampal neu rons. C handía A .1,2 , Bronfman F.C3, Lazo OM3, Álvarez A.R1,2. 1Laboratorio de Señalización Celular, 2CARE-Chile-UC, 3Departamento de Ciencias Fisiológicas, Pontificia Universidad Católica de Chile. [email protected] (29) E xp ression of dopamine receptor D3 on C D4 + T-cells favors T h1 immunity. F rancisco Contreras1,2, Carolina Prado1,2, Hugo González1,2, Dafne Franz1, Daniela Elgueta1, Rodrigo Pacheco1. 1 Fundación Ciencia & Vida, 2Universidad Andrés Bello. [email protected] (31) F unctional characterization of ca rgo binding sites on the μ2-subunit of adaptor protein complex 2. Esteban Corales, Yimo Lin, Patricia V. Burgos, Gonzalo A. Mardones. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile. [email protected] (33) E valuation of Synaptotagmin-I involvement in saliva ry gland hypofunction of Sjögren's Syndrome patients. C haracterization of C a2+ signaling in 3D acini. Cortés J, Hidalgo J, Bahamondes V, Urra H, Barrera MJ, Castro I, Aguilera S, González S, Molina C, Leyton C, Urzúa U, Sepúlveda D and González MJ. ICBM-Facultad de Medicina, Universidad de Chile. [email protected] (35) A ltered exp ression of SL A M family receptors and thei r adaptor p roteins in lupus-p rone mice. N. C risóstomo1, A. Gleisner1, M. Rosemblatt1,2,3, M.R. Bono1. 1Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Fundación Ciencia &Vida. 3Universidad Andrés Bello. [email protected] (37) G alectin-8 regulates the homeostasis of T and B lymphocytes. C ristobal C u r kovic1,2*, Evelyn Pardo1,2*, David Valenzuela3, Alfonso González1,2 and Andrea Soza1,2. Centro de Envejecimiento y Regeneración (CARE), Fac. Ciencias Biológicas1. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina2. Pontificia Universidad Católica de Chile. Regeneron Pharmaceuticals Inc, N.Y. USA3. *Pardo E. and Curkovic C. contributed equally to this study. [email protected] (39) Novel glucocorticoid-response elements in st2 distal p romoter a re responsible for transcriptional activity in mast cells. Díaz-Jiménez D. 1, Sepúlveda H 2, Montecino M.2, Quera R.3, Hermoso MA1. 1Innate Immunity Lab, Immunology Program, ICBM, Faculty of Medicine, Universidad de Chile. 2Center for Biomedical Research and FONDAP Center for Genome Regulation, Universidad Andres Bello. 3Gastronterology Unit, Clínica Las Condes, Chile. [email protected] (41) G lucocorticoid receptor localization in the diabetic kidney. Romina Bertinat 1, Karen Jaramillo1, Pamela Silva1, Rodrigo Gatica1, Francisco Nualart2, Alejandro Yáñez1. 1Instituto de Bioquímica y Microbiología, Universidad Austral de Chile. 2Departamento de Biología Celular y Centro de Microscopía Avanzada (CMA BIO-BIO), Universidad de Concepción. [email protected] (43) Effect of 5’-methylthioadenosine on C D4+ T cell differentiation towards T reg and T h17 cells. C ristian Doñas, Macarena Fritz, Paz Reyes, Alejandra Loyola and Mario Rosemblatt. Fundación Ciencia para la Vida, Universidad Andrés Bello. [email protected] (45) M n-Superoxide dismutase modulates cytotoxicity induced by Senecio sp (Chachacoma) in breast cancer cells under hypoxia. C arlos E chiburú-C hau, Cristian Salas, Mauricio Cuéllar, Susana Alfaro, Juan Pablo Ogalde and Gloria Calaf. Instituto de Alta Investigación, Universidad de Tarapacá, Arica, [email protected] (Sponsor: G. Cabello). (47) Inflammation alters the exp ression of i ron transporters D M T1, FPN1 and Z IP14 in neurons, astrocytes and microglia. Urrutia P., Aguirre P., Esparza A. and Núñez MT. Department of Biology, Faculty of Science, University of Chile. [email protected]

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(49) Prima ry cilium ma r kers disappear as oligodendrocyte p recu rsor cells differentiate and become unresponsive to Shh. 1,2 Paulina F alcón U, 1Carlos Carrasco P, 1Alejandro Roth M. & 2Verónica Palma A. Departamento de Biología, Facultad de Ciencias, Universidad de Chile. [email protected] (51) A ntioxidant activity of the Bios-p peptide analogue in H E K 293T cells. Pamela F ernández1,2, Ignacio Jofre1, Patricia Navarrete1, Magdalena Cuevas1, Ximena Romo2, Jorge Parodi3, Fernando Romero1. 1Center of Neurosciences and Peptides Biology- CEBIOR-BIOREN-CEGIN, Universidad de La Frontera, Temuco, Chile. 2Departamento de Ciencias Biológicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Sede Concepción, Chile. 3Laboratorio de Fisiología de la Reproducción, Núcleo de Producción Alimentaria, Escuela de Medicina Veterinaria, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile. [email protected] (53) G alectin-8 expression and chemotherapeutic drug resistance in ova ry cancer cells. F elipe G aray1,2,3 , Felipe Leiva3, Alfonso Gonzalez1,2, Andrea V. Leisewitz3, Andrea Soza1,2. Centro de Envejecimiento y Regeneración (CARE)1, Facultad de Ciencias Biológicas, Departamentos de Inmunología Clínica y Reumatología2 y Hematología-Oncología3, Facultad de Medicina, Pontificia Universidad Católica de Chile. [email protected] (55) Bci-1: a new tissue specific, negative regulator of autophagy. G herardelli C 1, Vicencio E1, Matamala JM2, Rojas M1, Hetz C2, and Manque P1. 1Center for Genomics and Bioinformatics, Universidad Mayor, 2Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Chile. [email protected] (57) A LS ast rocytes release factors that trigger c-A bl phosphorylation in motoneu rons: role of sodium channel activity and oxidative st ress. David Gonzalez1, Fabiola Rojas1,2, Elsa Fritz1,2, Alexis Martinez3, Sebastian Abarzua1, Nicole Cortes1, Alejandra Alvarez3, Brigitte van Zundert1. 1Andrés Bello 2 3 University; University of Concepción; P. Universidad Católica de Chile. [email protected] (59) Participation of monoclonal antibodies in detection and study of RSV-induced pathogenesis through FcγRs.  Roberto Gómez1, Kelly Cautivo1 and Alexis Kalergis1,2. 1Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina, Pontificia Universidad Católica. [email protected] (61) Pola rization of C x43 towa rds the immunological synapse induces N K -mediated tumor cell death by granzyme-B and calcium dependent mechanisms G uer rero I., Tittarelli A., MendozaNaranjo A., Farías M., Salazar-Onfray F. Tumor Immunology Laboratory, Faculty of Medicine, University of Chile. [email protected] (63) N-terminus domain cont ributes to thermal and chemical sensitivity of T RPM8 channel. H ardy, P.1, van Zundert, B.2, Madrid, R.1, Viana, F.3 and Pertusa, M.1. 1Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile. 3Centro de Investigaciones Biomédicas, Universidad Andrés Bello, 2Instituto de Neurociencias de Alicante, UMH-CSIC, España. [email protected] (65) T wo-dimensional elect rophoresis of different culture conditions of the fish pathogen Streptococcus phocae . C h ristian H idalgo, Rodolfo Paredes, Julio Retamales and Rubén AvendañoHerrera. Universidad Andrés Bello. [email protected] (67) A dult guinea pig brain has a lateral ventricle extention involved in neurogenesis. Jara, N., Rodríguez, F., Oyarce, K. and Nualart, F. Neurobiology and Stem Cells Laboratory, Department of Cell Biology, University of Concepción. [email protected] (69) E valuation of a C hilean ext ract of Ugni molinae Turcsz, generator of vasodilation in rat aortas and antioxidant effect in C R L1730 cell line. Ignacio Jofré1 , Javier Retamal1, Erick Scheuermann2, Jorge Parodi3, Fernando Romero1. 1Center of Neurosciences and Peptides Biology-BIOREN-UFRO.

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Center of Food Biotechnology and Bioseparations-BIOREN-UFRO,3Laboratory of Reproductive Physiology, UCT. [email protected] (71) E xp ression and pola rized dist ribution of the cell su rface p rotein ta rget of anti-P autoantibodies from lupus patients. A ngel Ju rado-Tor res1,2, Fabián Segovia1,2, Marcela Bravo-Zehnder M 1,2, Carlos Céspedes2,3, Carlos Vio2,3, Loreto Massardo L1, David Valenzuela3, and Alfonso González 1,2. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina1. Centro de Envejecimiento y Regeneración2 and Departamento de Fisiología3, Fac. Ciencias Biológicas. Pontificia Universidad Católica de Chile. Regeneron Pharmaceuticals Inc, N.Y. USA3. [email protected] (73) T hymic st romal lymphopoietin is produced by human metapneumovi rus-exposed human airway epithelial cells. M arga rita K . L ay, Pablo Céspedes, Alexis M. Kalergis. 1Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, 2Departamento de Reumatología, Facultad de Medicina, P. Universidad Católica de Chile. [email protected] (75) Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system. Lopez-V er rilli M . A .1, Court F.A.1,2. 1Millennium Nucleus in Regenerative Biology, Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected], [email protected] (77) Plasticity of T reg and T h17 cells during immune response and tolerance induction. G . Tejón 1,2, V. Manríquez1, J. R. Mora2, M.R. Bono1, M. Rosemblatt1,3,4. 1Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School. 3Fundación Ciencia & Vida. 4Universidad Andrés Bello. [email protected] (79) T he kynase c-A bl is activated in A myot rophic L ateral Sclerosis motor neu rons and pa rticipates in neurodegeneration in the SO D1 G86R mice. A lexis M artínez1,2, Cristián Valls1,2, Brigitte Van Zundert3, Claudio Hetz4, Alejandra Álvarez1,2. 1Laboratorio de Señalización Celular, 2CARE-Chile-UC, Pontificia Universidad Católica de Chile. 3Center for Biomedical Research. University Andres Bello. 4 Laboratory of Cellular Stress and Biomedicine, University of Chile. [email protected] 13:00 – 14:00

Plena ry Lectu re Sociedad de Biologia C elula r de C hile Volcanes Room C hair: C hristian Gonzalez-Billaut Regulation of memb rane p rotein t rafficking during neu ronal pola rization. A lfredo C áceres, Instituto Investigación Médica Mercedes y Martin Ferreyra (Inimec-Conicet), Universidad Nacional de Cordoba, Argentina.

14:00 – 15:30

L unch

15:30 – 16:30

Plena ry Lectu re C entro de Investigaciones Biomédicas y C entro F O N D A P de Regulación del G enoma, Universidad Nacional A nd res Bello Volcanes Room C hair: G iancarlo de Fer ra ri The genetics of Parkinson’s disease: moving from va riant to function. A ndrew Singleton. Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, USA. (for the International Parkinson’s Disease Genomics Consortium) [email protected]

16:00 – 18:30

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

16:30 – 18:30

Poster V iewing Session I: 1-80 E ven Numbers Convention C enter Foyer Coordinators: M arcela B ravo – G areth O wen – Rodolfo Pa redes

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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(2) Reprimo, a potential biomar ker for p rospective clinical t rial in gast ric cancer. M aturana M J., Olivares W., Garcia E., Torres V., Padilla O., Aguayo F., Calvo A., Ferreccio C., Corvalán AH. Pontificia Universidad Católica de Chile. [email protected] (4) C ardiac Rho kinase activation ea rly after experimental acute myoca rdial infa rction. C laudia M era, María Ocaranza, Renato Ramírez, Soledad Veliz, Iván Godoy, Jorge Jalil. Departamento de Enfermedades Cardiovasculares, Laboratorio de Cardiología Molecular, Escuela de Medicina, P. Universidad Católica de Chile. [email protected] (Sponsor: S. Lavandero). (6) Jitterbug(jbug)/F ilamin and protein tyrosine phosphatase 69D (PT P69D) are required fo r tendon cell adaptation to mechanical stress and axon growth. C laudia Molina and Patricio Olguín. Programa de Genética Humana, ICBM, Facultad de Medicina, Universidad de Chile. [email protected] (8) Role of Panx1 hemichannels in the secretory activity of neuroendocrine ch romaffin cells. F anny Momboisse1 , Catherine Estay1, María J. Guerra1, Ximena Baéz1, Juan C. Saez2, Agustin D. Martínez1 and Ana M. Cárdenas1. Centro Interdisciplinario de Neurociencias de Valparaíso, 1Universidad de Valparaíso, Valparaíso, and 2Departamento de Fisiología, P. Universidad Católica de Chile, Santiago. [email protected] (10) Release of gliotransmitters th rough astroglial connexin 43 hemichannels is necessa ry for fea r memory consolidation in the basolateral amygdala. Jimmy Stehberg, Rodrigo Moraga-A ma ro, Cristián Salazar, Álvaro Becerra, Cesar Echeverría, Juán Andrés Orellana, Geert Bultynck, Raf Ponsaerts, Luc Leybaert, Felipe Simon, Juán Carlos Sáez, Mauricio Retamal. Universidad Nacional Andrés Bello (Chile), Universidad del Desarrollo (Chile), Pontificia Universidad Católica de Chile (Chile), Katholieke Universiteit Leuven (Belgium), Ghent University (Belgium). [email protected] (12) A ctivation of signalling pathways in response to spinal cord injury in Xenopus. M auricio Moreno1, Dasfne Lee1, Leonardo Almonacid2, Francisco Melo2, Juan Larraín1 . 1Center for Aging and Regeneration and Millennium Nucleus in Regenerative Biology 2Molecular Bioinformatics Laboratory, Millennium Institute on Immunology and Immunotherapy. Pontificia Universidad Católica de Chile. [email protected] (14) I L-6 muscle levels a re elevated in cachexia using a murine model of cancer. Nei ra J., Espinoza A., Lobos L., Quest AFG, Jaimovich E. Centro de Estudios Moleculares de la Célula, ICBM, Facultad de Medicina, Universidad de Chile. jordana.nf @ gmail.com (16) Differential patterns of RPR M exp ression throughout different multistep p rogression of gastric cancer. O liva res W 1, Gonzalez C1, Maturana MJ1, Cerda J1, Montecinos V1, Aguayo F2, Corvalán AH1. 1 Hematology-Oncology Department, P. Universidad Católica de Chile & 2Virology Department, U. Chile. [email protected] (18) Neogenin- 1 mediates angiogenesis induced by non-classical angiogenic factors produced by human  Wharton’s  Jelly  mesenchymal  stem  cells  (WJ-MSC). C arolina O rtiz1, Cynthia Villarroel1, Catalina Prieto1, Rodrigo Pinto2, Verónica Palma1. 1Laboratory of Stem Cells and Development. Faculty of Sciences, University of Chile. 2Laboratory of Stem Cells & Tissue Regeneration, Faculty of Biological Sciences, PUC. [email protected] (20) C aveolin-1  and  β-catenin levels a re increased in L PS-stimulated dendritic cells. C ésa r O yarce1,2, Andrés Herrada1, Nicolás Bustamante1, Andrew Quest2 and Álvaro Lladser1. 1Laboratory of Gene Immunotherapy, Fundación Ciencia & Vida. 2Laboratory of Cellular Communication, Universidad de Chile. [email protected] (22) P r e n a t a l-st r e ss a f f e c t s R e e l i n a n d p 35 e x p r e ssi on i n vi vo a n d i n vi t ro : A co n n e c t ion b e t w e e n st r e ss r esp o n se a n d mo le c u l a r si gn a l i n g i n vol v e d i n n e u r on a l p l a st i c i t y . P a l a c i os- G a r c í a I sm a e l1,2, Díaz-Véliz Gabriela3, Abo it iz Francisco2, Gonzalez-Billault Christ ian1. 1Laboratory of Cell and Neuronal Dynamics, Department of Bio logy, Facult y of Sciencies, Universidad de Chile. 2Centro de Invest igación Médica, Pontificia Universidad Católica. 3Programa de Farmaco logía Mo lecular y Clínica, Instituto de

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Invest igació n Bio médica, Universidad de Chile. ipalacio [email protected] (24) Sodium-dependent vitamin C transporter 2 (SV C T2) t rafficking is altered in H untington’s disease. 1 A lejand ra Pa r ra, 1Aníbal Acuña, 1Adriana Covarrubias, 1Macarena Solís, 2Claudio Hetz, 1Ilona Concha and 1Maite Castro. 1Instituto Bioquímica y Microbiología, UACh, 2ICMB, U. Chile. [email protected] (26) Beneficial effect of atorvastatin on nitric oxide and reactive oxygen species p roduction by human endothelial cells exposed to cocaine or plasma from cocaine addicts. Perei ra-F lores K , Ebensberger R, Mezzano D, Pereira J, Sáez CG. Pontificia Universidad Católica de Chile, Santiago, Chile. [email protected] (28) W eb-based platform for biomedical ground truth. Raquel Pezoa 1,2, Victor Castañeda1, Steffen Härtel1. 1SCIAN-Lab/CEDAI SpA at BNI & Faculty of Medicine, University of Chile, 2CCTVal-UTFSM. [email protected] (30) G ene exp ression of neu rot rophic factors and related molecules after st roke. C laudia Pissani 1,2*, Katherine Stack1,2, Gareth I. Owen1, Francisca C Bronfman1,2. 1Physiology Department, Pontificia Universidad Católica de Chile. 2Millennium Nucleus in Regenerative Biology (MINREB). [email protected] (32) Steroid regulation of the Protease A ctivated Receptor 4 (PA R4) in breast cancer. Ramí rez C . 1,2, Orihuela P.1, Owen G.I2, Oliva B. 2, González P.2, Bravo M.L.2. 1Facultad de Química y Biología, USACH. 2Facultad de Ciencias Biológicas, PUC. [email protected] (34) O verexp ression of D N A repai r glycosylases Tc O G G1 and TcN T H1 in Trypanosoma cruzi. Santiago Ramírez, Iván Ponce, Sofía Sepúlveda, Lucía Valenzuela, Soledad Sierra, Paula Bahamondes, Natalia Muñoz, Ulrike Kemmerling, Norbel Galanti, Gonzalo Cabrera. Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. [email protected] (36) Synchronizing basolateral t raffic from recycling endosomes by activating a phosphatidic acid/P K A pathway. C laudio Retamal and Alfonso González. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina. Centro de Envejecimiento y Regeneración (CARE). Fac. Cs. Biológicas. Pontificia Universidad Católica de Chile. [email protected] (38) N K T cells and regulatory T cells: players of the immunosupp ressive response observed in human gast ric cancer. C H Ribei ro, F Cristi, G Pérez, K Kramm, M Bustamante, M Tapia, C Hernández, JC Aguillón, and MC Molina. Programa Disciplinario de Inmunología (ICBM) and Departamento de Cirugía Digestiva, Hospital Salvador, Facultad de Medicina, Universidad de Chile, Santiago, Chile. [email protected] (40) C arbon monoxide decreases endosome-lysosome fusion and inhibits soluble antigen presentation by dendritic cells. Virginie Tardif4 , Leandro J. Carreño1, Séverine Remy4, Sebastián A . Riquelme1, Claudia M. Cortés3, Thomas Simon4, Marcelo Hill4, Cédric Louvet4, Claudia A. Riedel3, Philippe Blancou4, Jean-Marie Bach5, Ariel Savina6, Christine Chauveau4, Ignacio Anegon4 and Alexis M. Kalergis1,2. 1Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, 2Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. 3Millenium Institute on Immunology and Immunotherapy, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina Universidad Andrés Bello. 4Centre de Recherche en Transplantation et Immunologie, Université de Nantes, France. 5ONIRIS, France. 6Institut Curie, France. [email protected] (42) A n shR N A-based adjuvant against IκBα promotes antitumor T cell responses mediated by D N A vaccines . Jonathan Roco, Andrés Herrada, Alvaro Lladser. Laboratory of Gene Immunotherapy, Fundación Ciencia y Vida, Santiago, Chile. [email protected]. (44) C haracterization of T cell receptor Vβ  usage  in  response  to  the  respiratory  syncytial  virus.  H ernán G . Cor rea1, Bruno A. Ramirez1, Roberto S. Gomez1, Jorge E. Mora1 and Alexis M. Kalergis1,2.

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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1

Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, 2Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. [email protected] (46) Structural and functional cha racterization of ca rgo-binding sites for ty rosine-based signals on the µ1A-subunit of adaptor p rotein complex 1. B reyan Ross, Esteban Corales, Yimo Lin, Patricia V. Burgos, Gonzalo A. Mardones. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile. [email protected] (48) Remodeling of mitochondrial O X PH OS complexes by copper defficiency alters energetic output. L ina Ruiz, Erik Jensen, Rodrigo Bustos and Alvaro Elorza. Experimental Bioenergetics Laboratory, Universidad Andres Bello (UNAB). [email protected] (50) Role of mitochondria in neuronal C a 2+ and R OS signals induced by the A lzheimer´s diseaseassociated amyloid-β peptide oligomers. 1SanM artín C D, 1,2Hidalgo C, 1,3Paula-Lima AC. 1CEMC, BNI and 2ICBM; Facultad de Medicina; 3Facultad de Odontología. Universidad de Chile. (52) E volutionary origin of segment formation mechanisms in animals. Sar razin, A . F. 1,-3, Peel, A.D.2, Abdo Abou Issa, A.3, Manuel, M.3, Averof, M.2. Instituto de Química, P. Universidad Católica de Valparaíso1; IMBB, Heraklion, Greece2; UPMC, Paris, France3. [email protected] (Sponsor: N. Osses). (54) E G F RvII I-dependent cell growth in a glioblastoma cell line can be counteracted by inhibitors of phosphatidic acid phosphohydrolase. Ronan Shaughnessy 1,2, Carolina Otero1,2, Andrea Soza1,2, Alfonso Gonzalez 1,2. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina1, Centro de Envejecimiento y Regeneración (CARE), Facultad Ciencias Biológicas2, Pontificia Universidad Católica de Chile. [email protected] (56) E ffects of chitosan pa rticles on cell p roliferation in gingival fib roblasts. Silva D, Arancibia R, Cáceres M, Acuña C, Tapia C, Diaz-Dosque M, Martínez J, Smith PC. Odontología. Facultad de Medicina. Pontificia Universidad Católica de Chile, Facultad de Ciencias Químicas y Farmacéuticas e INTA, Universidad de Chile. [email protected] (58) Trypanosoma cruzi calreticulin, exogenously bound to epimastigotes cellula r memb ranes, promotes adhesion and penetration into host cells. Sosoniuk, E., Aguilar, L., Coddou, F., Maldonado, I., Orellana, J., Valck, C*., Ferreira, A*. Laboratorio de Inmunología de la Agresión Microbiana, PDI, ICBM, Facultad de Medicina, Universidad de Chile. [email protected] (60) R U N X2 promote angiogenesis in osteosa rcoma by modulating pro-angiogenic target genes. A ndrés Stevenson 1,2, Sofia Jerez1,2, Paola Casanello3, Gary Stein4, Andre van Wijnen5, Mario Galindo1,2. 1 Millennium Institute on Immunology and Immunotherapy, 2Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile. 3Instituto de Investigaciones Medicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile. 4Department of Biochemistry, Vermont Cancer Center for Basic and Translational Research, University of Vermont. 5Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic. [email protected] (62) F rizzled-9 mediates postsynaptic matu ration at the verteb rate neu romuscula r junction. Jaime Teneb, Juan Pablo Henríquez*. Department of Cell Biology and CMA Bio-Bio, University of Concepcion, Concepcion, Chile. *[email protected] (64) Diffusion and cell-su rface dynamics of T RPM8-containing vesicles is affected by agonist stimulation in a positive feedback loop. C arlos A . Toro, Deny Cabezas and Sebastián Brauchi. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia. (66) T he protein disulfide isomerase family member E Rp57 modulates p rion p rotein turnover. Tor res M . 1,2,3, Woehlbier U.1,2,3, Andreu C.1,2,3, Solda T.4, Molinari M.4, Hetz C.1,2,3. 1Biomedical Neuroscience Institute, 2Center for Molecular Studies of the Cell, Faculty of medicine, University of

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Chile, and 3Neurounion Biomedical Foundation, Santiago, Chile, 4Institute for Research in Biomedicine, Bellinzona, Switzerland. [email protected] (68) Imatinib decrease the exp ression of B A C E1. C hamor ro D.1,2, Estrada LD. 1,2 and Alvarez A.R.1,2. 1 Laboratorio de Señalización Celular, Depto. de Biología Celular y Molecular, Facultad de Ciencias Biológicas, 2CARE-Chile-UC, Pontificia Universidad Católica de Chile. [email protected] (70) A possible role for astrocytes in the neuroprotective effect of ketone bodies. Rocío V aldebenito and L. Felipe Barros. Centro de Estudios Científicos (CECs), Valdivia, Chile. [email protected] (72) SC O-spondin from emb ryonic cerebrospinal fluid is requi red for neu roepithelial differentiation. V era A, Stanic K, Gonzalez M, Montecinos H, Caprile T. Departamento Biología Celular, Universidad de Concepción. [email protected] (74) PRP K and Rab35 regulate axon elongation during development. V illa r roel D 1 and GonzálezBillault C1. 1Cellular and Neuronal Dynamics Laboratory, Department of Biology, Faculty of Sciences, Universidad de Chile, Santiago, Chile. [email protected] (76) C alcium release from the endoplamic reticulum after injury t riggers axonal degeneration. V illegas R. 1 and Court, F.A.1,2. 1Millennium Nucleus in Regenerative Biology, (MINREB), Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected], [email protected] (78) Loss of Drosophila RhoG E F3 causes defects in saliva ry gland migration. A lejand ro Zúñiga, and Verónica Cambiazo. Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile & Fondap Center for Genome Regulation. [email protected] (80) G estational thyroid hormone deficiency affects the role of ast rocytes on neu ronal development, synapsis formation and immunogenicity in the offsp ring. K atherine A cevedo1, Gabriela Zúñiga1, Pablo Cisternas1, Betsi Flores1, Cecilia Opazo1, Leandro J. Carreño3, Helene Boudin4, Susan Bueno2, Alexis M. Kalergis2 and Claudia A. Riedel1. 1F. de Ciencias Biológicas, U. Andrés Bello. 2Departamento de Genética Molecular y Microbiología, F. de Ciencias Biológicas, P.U. Católica de Chile. Instituto Milenio de Inmunología e Inmunoterapia. 3Albert Einstein College of Medicine, New York, USA. 4 INSERM UMR643, CHU Nantes, Nantes, France. [email protected] 18:30 – 20:30

O ral Presentations I Volcanes Room C hair: B rigitte van Zundert and Co-C hair: M anuel K ukuljan Regulation of PSD95 gene exp ression du ring development and memory formation. Fernando J Bustos1,3,4, Rodrigo Aguilar3,4, Berta Henríquez3, Rodrigo Moraga3, Jimmy Stehberg3, Marianne Rots2, Martín Montecino3,4, Brigitte van Zundert3. 1University of Concepción; 2UMCG, The Netherlands; 3Center for Biomedical Research, Andres Bello University; 4FONDAP C.G.R. [email protected] W nt5a regulates microR N As in hippocampal neurons. Juan F. Codocedo, Nibaldo C. Inestrosa. Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. [email protected] C tip1/Bcl11a is requi red for the development of corticospinal p rojection neu rons. C ánovas J.1, Berndt F.A.1, Sepúlveda H.2, Aguilar R.2, Montecino M.2, Sierralta J.1, Kukuljan M.1. 1Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile. 2Center for Biomedical Research, Faculty of Biological Sciences, Universidad Andrés Bello. [email protected] C aveolin-1-enhanced metastatic cancer cell migration is mat rix dependent and requi res tyrosine-14. Rina O rtiz1, Vicente Torres1,2, María Inés Díaz1, Justin Cooper-White3, Víctor Castañeda1, Steffen Härtel1, Lisette Leyton1 and Andrew F.G. Quest1. 1Center for Molecular Studies of the Cell, Faculty of Medicine, Universidad de Chile. 2Department of Basic and Communitarian Sciences, Faculty of Dentistry, Universidad de Chile, 3Univerity of Queensland, Australia. [email protected]

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Innate immune response activated by adherent-invasive Escherichia coli st rains isolated from C rohn´s disease patients. 1 M arjorie De la F uente, 3Rodrigo Quera, 2Roberto Vidal, 1Marcela Hermoso. 1 Immunology and 2Microbiology Program, ICBM, Faculty of Medicine, Universidad de Chile. 3 Gastroenterology Unit, Clínica Las Condes, Chile. [email protected] Networ k-based pathway analysis of genome wide associations studies in A lzheimer's disease. E duardo Pérez-Palma, Bernabé Bustos, Camilo Villamán, Marcelo Alarcón, Miguel Avila, Alexis Peralta, Matias Medina, Giorgia Ugarte and Giancarlo V. De Ferrari. Center for Biomedical Research, Universidad Andrés Bello, Santiago, Chile. [email protected] T y r-couma rin functional incorporation into p roteins synthesized by H E K 293T cells. X . Steinberg°, J. Villegas°, J. Galpin*, S. Goodchild*, C. Ahern* and S. Brauchi°*. University of British Columbia* and Universidad Austral de Chile°. MECESUP [email protected] Following a glycolitic enzyme inside exosomes. A lejandro L ua rte, Javier Rubio, Ursula Wyneken. Universidad de los Andes. [email protected] 20:30

Dinner

22:00 – 23:30

T ribute to Federico Leighton Puga Volcanes Room C hair: Nibaldo C . Inestrosa Best T heses A wa rds F undacion C hilena para Biologia C elula r Volcanes Room C hair: A rtu ro Y udelevich and Co-C hair: M aria Rosa Bono

Undergraduate V icente V alenzuela Patera kis, M aster in Biological Sciences, Universidad de C hile. F unción del estrés de retículo endoplasmático y la respuesta a p roteinas mal plegadas en la recuperación locomotora frente a una hemisección a la medulla espinal. T hesis A dvisors: C laudio H etz, U. de C hile and Felipe Court, P. U. C atólica de C hile Graduate Leona rdo V aldivia A lva rez, PhD in Sciences, Universidad de C hile Identificación de genes esenciales pa ra el desa r rollo de la línea lateral y la diferenciación de las células mecanosensoriales en el pez ceb ra. T hesis A dvisor: M iguel A llende, U. de C hile

Chilean Society for Cell Biology – XX VI Annual Meeting – October 23-27, 2012

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T H U RSD A Y, O C T O B E R 25, 2012 08:00

Poster Mounting Session I I: N° 81 to N° 159 Convention C enter Foyer

09:00 – 11:00

Symposium F O N D E C Y T 1095089 and 1100137 C albuco Room C hair: C hristian Gonzalez-Billaut and Co-C hair: A ndrés Couve “F unctional Intracellula r Dynamics” Molecula r mechanism of filopodia formation. Sohail A hmed. Institute of Medical Biology, Singapore 138678. A ctin-dependent nuclear positioning contributes to cell pola rity in migrating cells. G regg G. G undersen, Eric S. Folker and Wakam Chang. Department of Pathology & Cell Biology, Columbia University, New York, NY. Role of E R shape and dynamics in regulating mitochondrial morphology. G ia Voeltz. Department of Molecular, Cellular & Developmental Biology, University of Colorado Boulder, USA. Symposium C enter for Molecula r Studies of the C ell (C E M C) and Department of Basic Sciences, F aculty of Dentist ry, Universidad de C hile T ronador Room C hair: A ndrew Q uest and Co-C hair: V icente A. Tor res “T umor C ell M igration and M etastasis” A non-canonical role for A rhgef28 in facilitating early focal adhesion kinase (F A K ) activation at cell adhesion sites. Nichol L. G. Miller, Christine Lawson, Sean Uryu, and David D. Schlaepfer. Moores University of California San Diego Cancer Center, La Jolla, CA USA. [email protected] O n the role of C aveolin-1 in tumor cell migration and metastasis. A ndrew F. G . Q uest. Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula (CEMC), Facultad de Medicina, Universidad de Chile. [email protected] Unexpected roles for a caspase in cell migration. Dwayne Stupack. Department of Reproductive Medicine, Moores UCSD Cancer Center, La Jolla, CA, USA 92093. [email protected] Role of Rab5 in focal adhesion dynamics and tumor cell migration, V icente A . Torres. Department of Basic and Communitarian Sciences, Faculty of Dentistry, Universidad de Chile, Santiago, Chile. [email protected]

10:30 – 13:00

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

11:00 – 13:00

Poster V iewing Session I I: 80-159 O dd Numbers Convention C enter Foyer Coordinators: M arcela H ermoso – Julieta González – Julio C ésa r C á rdenas (81) Neuronal transport of ascorbic acid is impai red in H untington'disease. 1 A níbal I. A cuña, 1 Magdalena Esparza, 1Felipe A. Beltrán, 1Carlos Kramm, 3Carlos Cepeda, 4Claudio Hetz, 1Ilona I. Concha, 2Sebastián Brauchi, 3Michael Levine, 1Maite A. Castro. 3Semel, UCLA; 4ICBM, U. Chile; 1I. Bioquímica y Microbiología, 2I. Fisiología, UACh. [email protected].

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(83) H ypothyroidism during p regnancy increases the severity of experimental autoimmune encephalomyelitis in the adult offsp ring. 1,2 E duardo A lbornoz, 2,3Tomás Rivero, 2,3Janyra Espinoza 2,4 Leandro Carreño, 1,2Claudia Cortes, 1,2Pablo Gonzalez, 1,2Pablo Cisternas, 2Kelly Cautivo, 2Tamara Catalán, 4Eliseo Eugenin, 4Joan Berman, 2,3Susan Bueno, 2,3Alexis Kalergis and 1,2Claudia Riedel. 1 Facultad de Ciencias Biológicas Universidad Andrés Bello. 2Millennium Institute on Immunology and Immunotherapy. 3Facultad de Ciencias Biológicas. Pontificia Universidad Católica de Chile. 4Albert Einstein College of Medicine New York. [email protected] (85) Pharmacologic induction of E R B B2 receptor endocytosis. M aría José A pud, Claudio Retamal, Andrea Soza, Alfonso González. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina, Centro de Envejecimiento y Regeneración (CARE), Fac. Ciencias Biológicas, Pontificia Universidad Católica de Chile. [email protected] (87) Immunomodulatory effect of M esenchymal Stem cells on T reg/T h17 balance is I L -6 dependent. A rgaluza M . F., Contreras R., Vega A.M., Fernández X., Bravo-Alegría J., Ibáñez C., Kurte M., Fuentealba R., Carrión F. Laboratorio de Inmunología Celular y Molecular, Universidad de los Andes. [email protected] (89) Netrin-4 is important for neu roblastoma survival th rough inhibition of p ro-apoptotic activity of Neogenin-1, a mediator of SH H activity in cancer. A ndrea A r ros1, Natalie Espinoza1, Luis A. Milla1, Verónica Palma1. 1Laboratory of Stem Cells and Development. Faculty of Sciences, University of Chile. [email protected] (91) Oscillatory glycolytic switch in neurons. 1,2 Felipe Baeza-Lehnert, 1,2Robin Gutiérrez and 1L. Felipe Barros. 1Centro de Estudios Científicos (CECs), Valdivia, Chile & 2Universidad Austral de Chile, Valdivia, Chile. [email protected] (93) E xpression of PD-L1 on dendritic cells modulate the proliferation and differentiation to subpopulations of t cells in vitro. Behrens C 1,2, Falcón C1,2, Tempio F1,2, Pereda C1,2, Salazar-Onfray F1,2, López MN1,2. 1Millennium Institute on Immunology and Immunotherapy; 2Disciplinary Program of Immunology, ICBM, University of Chile, Santiago, Chile. [email protected] (95) T urnover of C99 requi res incorporation into multivesicula r bodies mediated by ESC R T machinery but independent of its ubiquitination. Hianara Bustamante, Andrés Rivera-Dictter, Viviana Cavieres, Alexis González, Vanessa Muñoz, Gonzalo A. Mardones and Patricia V. Burgos. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. [email protected] (97) M as-1 receptor exp ression is regulated in skeletal muscle at rophy. Vázquez, Y., Morales, MG., C abello-V er rugio, C . Laboratorio de Biología y Fisiopatología Molecular, Facultad de Ciencias Biológicas, Universidad Andres Bello. [email protected] (99) 2'-7'-Dichlorodihydrofluorescein diacetate: a good indicator of int racellula r reactive oxygen species? 1Pino JA, 1 C armona E , 1Reyes JG. 1Instituto de Química, P. Universidad Católica de Valparaíso, Chile. [email protected] (101) A G PA T2 deficiency determines impai red adipogenic differentiation and abnormal lipid droplet st ructu re in mouse emb ryonic fibroblasts. K elly C autivo1,2 and Víctor Cortés1. 1Department of Nutrition, Diabetes and Metabolism, School of Medicine, Pontificia Universidad Católica de Chile. 2 Doctoral Program in Biological Sciences, Microbiology and Molecular Genetics, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile. [email protected] (103) O ptical flow for motion estimation and tracking of subcellula r, cellula r and sup racellula r dynamics. M auricio C erda 1, Jorge Jara1,4, Alex Córdova1, Jorge Toledo1,2, Eduardo Pulgar3, CarmenGloria Lemus3, Omar Ramírez1, Jarno Ralli1,5, Miguel Concha3, Steffen Härtel1. 1SCIAN-Lab, 2CouveLab and 3LEO-Lab at BNI & Faculty of Medicine; 4DCC; University of Chile. 5University of Granada. [email protected]

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(105) Dehydroascorbic acid modulates glycolytic, pentose-phosphate pathway and glutathion activity in cortical neu rons. Ped ro C isternas, Elena Uribe, Karina Oyarce, Paula Llanos, Carmen SilvaAlvarez and Francisco Nualart. Department of Cell Biology, University of Concepcion. [email protected] (107) Possible topology of neu ronal su rface P antigen involved in neuropsychiatric lupus. M arcela B ravo- Zehnder 1,2 , Fabián Segovia-Miranda1,2, Francisca Barake1, Loreto Massardo2, Alfonso González1,2. Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas1, Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina2, Pontificia Universidad Católica de Chile. (109) Niemann Pick type C models show increased levels of H D A C2 mediated by the c-A bl kinase. Contreras P. 1,2, González Z.M.1,2, Yañez M.J.1,2,3, Zanlungo S.3, Alvarez A.R.1,2. 1Laboratorio Señalización Celular, 2CARE-Chile-UC, 3Escuela de Medicina, Pontificia Universidad Católica de Chile. [email protected] (111) T he E R st ress factor X BP1 controls secretion of amyloid- in experimental models of Alzheimer’s  Disease.  V íctor H ugo Cornejo1,2, Peter Thielen1,2, René Vidal1,2, Claudia Molina1,2, Gabriela Martinez1,2, Andrea Paula-Lima1,2, and Claudio Hetz1,2,3. 1Biomedical Neuroscience Institute, 2 Center for Molecular Studies of the Cell, Faculty of Medicine, Universidad de Chile and 3Neurounion Biomedical Foundation, Santiago, Chile. [email protected] (113) SRP K s exp ression in the rat C NS and the assembly of the active zones. Yocelin C ruz, Viviana Torres, Pedro Zamorano*. Faculty of Health Sciences, University of Antofagasta, Chile. *[email protected] (115) A tlastin is requi red for morphogenesis of the endoplasmic reticulum in Drosophila muscle cells. C ristian De G regorio1,2 , Guillermo Moya1,2, Jimena Sierralta1,2 and Andrés Couve1,2. 1 Program of Physiology and Biophysics, ICBM, Faculty of Medicine, University of Chile. 2 Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile. [email protected] (117) Identification and characterization of a novel protein complex of the trans-Golgi networ k (T G N). Priscilla Cortés1,2, Gonzalo Mardones, Claudia Oyanadel1,2, Claudio Retamal1,2 and Alfonso González1,2. Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas1, Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina2, Pontificia Universidad Católica de Chile. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile3. [email protected] (119) Rab5 activation is required for C aveolin-1 mediated cell migration, Rac1 activation and invasion of cancer cells. Jorge Díaz1,2, Pablo Mendoza2, Lisette Leyton1, Andrew F.G. Quest1, Vicente Torres2. 1FONDAP Center for Molecular Studies of the Cell, Faculty of Medicine, Universidad de Chile. 2 Laboratory of Cellular and Molecular Biology, Department of Basic and communitarian Sciences, Faculty of Dentistry, Universidad de Chile. [email protected] (121) C hromatin modifications du ring T reg and T h17 cell differentiation. Macarena Fritz, C ristian Doñas, Marjorie Reyes, Paz Reyes, Mario Rosemblatt, and Alejandra Loyola. Fundación Ciencia y Vida; Universidad Andrés Bello. [email protected] (123) C ellula r cha racterization of the response to spinal cord injury of Xenopus laevis. G abriela E dwards, Dasfne Lee-Liu, Mauricio Moreno, Felipe Court, Juan Larraín. Center for Aging and Regeneration, Millennium Nucleus in Regenerative Biology, Pontificia Universidad Católica de Chile. [email protected] (125) Impaired lea rning due to RSV infection. Janyra A . Espinoza 1, Claudia M. Cortes2, Javier A. Valenzuela3, Rodrigo A. Sandoval3, Pablo F. Céspedes1, Roberto S. Gomez1, Floria C. Pancetti3, Susan M. Bueno1,5, Claudia A. Riedel2,5 and Alexis M. Kalergis1,4,5. 1Millenium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2Millenium Institute on Immunology and Immunotherapy,

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Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina Universidad Andrés Bello. 3Laboratorio de Neurotoxicología Ambiental, Departamento de Ciencias Biomédicas, Facultad de Medicina, Universidad Católica del Norte. 4Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. [email protected] (127) E xogenous mucins induce p ro-inflammatory response. Ba r rera M J, Hermoso M, Castro I, Cortés J, Sepúlveda D, Aguilera S, Molina C, González S, Urzúa U, Langjahr P, Leyton C and González MJ. ICBM, Facultad de Medicina, Universidad de Chile. [email protected] (129) A xotomy induces a ret rograde signaling response that activates the c A M P response elementbinding (C R E B) transcription factor in compa rtmentalized cortical cultu res. C a rlos F lores, Andrés González and Francisca Bronfman. Physiology Department, Pontificia Universidad Católica de Chile. [email protected] (131) E R st ress and S T2 inflammatory pathway in intestinal goblet cells. 1 G arcia M ., 1Romero D., 2 González MJ, 3Quera R., 1Hermoso MA. 1Immunology and 2Cell Biology Program, ICBM, Faculty of Medicine, Universidad de Chile. 3Gastronterology Unit, Clínica Las Condes. Chile. [email protected] (133) O ptimizing the ret rovi ral t ransduction of T cells with chimeric antigen receptors (C A Rs) against the carcinoemb ryonic antigen (C E A) for a potential antitumor immunotherapy. Paula González-F igueroa 1, Paola Murgas1, Álvaro Lladser1. 1Laboratory of Gene Immunotherapy, 1Fundación Ciencia y Vida, Santiago, Chile. [email protected] (135) Differential gene exp ression and altered cell viability induced by inflammatory damage in astrocytes from offsp ring gestated in hypothyroxinemic mothers. 1 Pablo González, 1Ma. Cecilia Opazo, 1Eduardo Albornoz, 2, 3Leandro Carreño, 2Susan Bueno, 2Alexis Kalergis and 1, 2Claudia Riedel. 1 Laboratorio de Biología Celular y Farmacología, Facultad de Ciencias Biológicas, Universidad Andrés Bello. 2Instituto Milenio de Inmunología e Inmunoterapia. Pontificia Universidad Católica de Chile. 3 Albert Einstein College of Medicine, New York. [email protected] (137) F ibroblast-li ke synoviocytes from rheumatoid arthritis patients exp ress and secrete galectin-8. C laudia Gordillo D 1, Claudia Metz B2,3, Jorge Gordillo P2, Alfonso González2,3. Universidad de Chile1. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina2. Centro de Envejecimiento y Regeneración (CARE) Facultad de Ciencias Biológicas3. Pontificia Universidad Católica de Chile. [email protected] (139) R E C K a novel M M P inhibitor regulates the canonical W nt signaling through a syndecan-4 dependent mechanism in muscle p recu rsor cells. Jaime G utiér rez, Cristian Droppelmann, Enrique Brandan. Laboratory of Cell Differentiation and Pathology, CARE. Department of Cell and Molecular Biology, Catholic University of Chile. Santiago, Chile. [email protected] (141) Role of Src kinase-subst rate cortactin in cortical actin organization and exocytosis in bovine chromaffin cells. V alentina H aro, María J. Olivares, Catherine Estay, Arlek González, María J. Guerra, Ximena Baéz, Fanny Momboisse, Ana M. Cárdenas. Centro interdisciplinario de Neurociencia de Valparaíso, Universidad de Valparaíso. [email protected] (Sponsor: J. Ewer). (143) C haracterization of different B cell populations generated after immunization. Y essia H idalgo1, Leonardo Vargas1, María José Morales1, Mario Rosemblatt1,2,3, María Rosa Bono1. 1 Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Fundación Ciencia &Vida. 3 Universidad Andrés Bello. [email protected] (145) Copper overload modulates differentiation of eryth ropoietic cells in vivo. E ri k Jensen, Yancing Rossel, Alvaro Elorza. Experimental Bioenergetics Laboratory, Universidad Andres Bello. [email protected] (147) Dihydropyridine receptor (D H PR) regulates gene exp resion related to muscle plasticity. Jorquera G 1, Altamirano F1, Buvinic S1, Contreras-Ferrat A, Gentil C2, Garcia L2, Petri-Rouxel F2,

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Jaimovich E1, Casas M1. 1CEMC, Medicine Faculty, Universidad de Chile, Santiago-Chile. 2Institut de Myologie, Université Paris VI, Paris-France. [email protected] (149) Isolation and characterization of ova rian cancer initiating cells. K ato S.1, Bravo L.2, Gonzalez P.2, Barriga MI.1, Castellón EA3., Owen GI.2, Cuello M1. 1Division of Obstetrics and Gynecology, Faculty of Medicine, 2Department of Physiological Sciences, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, 3 Laboratory of Molecular and Cellular Andrology, University of Chile. [email protected] (151) Collateral sp routing and locomotor recovery after spinal cord injury is t riggered by axonal degeneration. Lemait re D.1, Collyer E.1, Catenaccio A.1, and Court, F.A.1,2. 1Millennium Nucleus in Regenerative Biology, (MINREB), Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected], [email protected] (153) Pharmacologic induction of E G F R endocytosis decreases proliferation of cancerous M N K 45 gastric cells. A lejandro López-González A .1,2 , Marcela Bravo-Zehnder1,2, Andrea Soza1,2, Alfonso González1,2. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina1, Centro de Envejecimiento y Regeneración (CARE), Fac. Ciencias Biológicas, Pontificia Universidad Católica de Chile2. [email protected] (155) Reprogramming antigen-specific T h17 and Regulatory T C ells in vitro. V . M anríquez 1 , G. Tejón1, P. Maldonado1, M.R. Bono1, M. Rosemblatt1,2,3. 1Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Fundación Ciencia & Vida. 3Universidad Andrés Bello. [email protected] (157) M ild mitochondrial uncoupling delays axonal degeneration in vitro and in vivo. M artinez N. W .1 and Court F.A.1,2. 1Millennium Nucleus in Regenerative Biology (MINREB), P. Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected], [email protected] (159) F asudil and atorvastatin reduce RhoA/R ho kinase pathway activation in human endothelial cells exposed to plasma from cocaine addicts. M endoza C, Pereira-Flores K, Pereira J, Mezzano D, Sáez CG. Pontificia Universidad Católica de Chile, Santiago, Chile. [email protected] 13:00 – 15:00

L unch

15:00 – 16:30

M inisymposium I I I “C ell Homeostasis and C ell F ate” C albuco Room C hair: Juan Pablo H enríquez and Co-C hair: Felipe Court Role of W nt/beta-catenin in pluripotency and differentiation of mouse emb ryonic stem cells. F. F aunes1, P. Hayward1 and Alfonso Martinez-Arias1. 1Department of Genetics, University of Cambridge, United Kingdom. [email protected] (Sponsor: J. Larraín) A xonal degeneration is assisted cell non-autonomously by glial cells. C atenaccio A . 1 and Court, F.A.1,2. 1Millennium Nucleus in Regenerative Biology, (MINREB), Catholic University of Chile and 2 Neurounion Biomedical Foundation. [email protected], [email protected] Relationships between the golgi and mitosis in Drosophila : role of p115. Iba r, C ., Cataldo, V. and Glavic, A. FONDAP CRG - Facultad de Ciencias - Universidad de Chile. [email protected] Role of the SO X2+ cells in spinal cord regeneration in Xenopus laevis. M uñoz, Rosana; Edwards, Gabriela; Tapia, Karina; Farías, Marjorie and Larraín, Juan. Center for Aging and Regeneration, Millennium Nucleus in Regenerative Biology. Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Chile. [email protected] X BP-1 controls the homeostasis of tissue-resident dendritic cells. F abiola Osorio, Sophie Janssens, Bart Lambrecht. Unit of Immunoregulation; Department for Molecular Biomedical Research(DMBR); VIB-Ghent University; Belgium. (Sponsor: M.R. Bono). [email protected]

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Daam1 controls asymmetric morphogenesis of Pa rapineal-H abenulo-Interpenduncula r ci rcuit in zeb rafish. A licia Colombo1, Karina Palma1, Lorena Armijo1, Ailen Signore1, Camila Morales1, Néstor Guerrero1, Miguel Concha1. 1Institute of Biomedical Sciences, University of Chile, Santiago, Chile. [email protected] M inisymposium I V “C ell Physiology” T ronador Room C hair: Nelson Osses and Co-C hair: Pedro Zamorano A-oligomers decrease and altered A PP p rocessing in A lzheimer's disease by Imatinib treatment. Est rada L. D 1,2 , Chamorro D1,2, von Bernhardi R3, and Alvarez A.R1,2. 1Laboratorio de Señalización Celular, 2CARE-Chile-UC, 3 Escuela de Medicina, P. Univ. Catolica de Chile. [email protected] Unraveling the C a 2+ signaling pathways that mediate the neuronal dysfunctions induced by amyloid-β peptide oligomers. Paula-L ima, A . C . 1, San Martin2, C., Baratini, P.2, Perez-Luco, T. 2, Plaza, J. 2, Adasme T.2 and Hidalgo C.2. 1Faculty of Dentistry, 2Faculty of Medicine, Universidad de Chile. [email protected] Different models for ch ronic and t ransitory skeletal muscle fibrosis. C ab rera D. 1 , Morales MG.1, Acuña MJ.1, Cabello-Verrugio C.2, Santos R.3, and Brandan E.1. 1Catholic University of Chile, Chile. 2 Andres Bello University, Chile. 3Minais Gerais Federal University, Brazil. [email protected] C hronic administration of nifedipine reduces resting calcium concent ration and damage in dystrophic mdx muscles. Altamirano F, López JR, Henríquez C, Valladares D, Casas M, Allen PD, Jaimovich E. CEMC, ICBM, Facultad de Medicina, Universidad de Chile. Chile. Brigham and Women’s  Hospital, Harvard Medical School, USA. [email protected] M I C U1 is a essential gatekeeper for M C U-mediated mitochondrial C a 2+ uptake that regulates cell survival. C a rdenas C .1,2, Mallilankaraman K.3, Doonan P.3 , Mak D.2, Foskett J.K.2, and Madesh M.3. 1 Anatomy and Developmental Biology Program, Institute of Biomedical Sciences, University of Chile, Santiago, Chile, 2Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA, 3Department of Biochemistry, Temple University, Philadelphia, Pennsylvania, USA. [email protected] A common minor groove shape readout mechanism is sha red by p roteins from different D N Arepai r pathways. Juan José Cifuentes and F rancisco M elo. 1Molecular Bioinformatics Laboratory, Millennium Institute on Immunology and Immunotherapy; 2Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. [email protected] 16:00 – 18:30

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

16:30 – 18:30

Poster V iewing Session I I: 80-159 E ven Numbers Convention C enter Foyer Coordinators: M aria C armen Molina – A licia M initti – E lías Utreras (82) Control of dopaminergic neu ron su rvival by the unfolded protein response transcription factor X BP1. M ercado G . 1*, Valdés P.1*, Vidal R.1,2, Parsons G.3, Court F.4, Martinez A.1, Armentano D.3, Schneider B.5, Aebischer P.5, and Hetz C.1,2. 1Biomedical Neuroscience Institute, 2Neurounion Biomedical Foundation, Santiago, Chile, 3Genzyme Corporation, MA, USA, 4P. Catholic University of Chile, Santiago, Chile and 5Brain Mind Institute, Lausanne, Switzerland. *Equal contribution. [email protected] (84) RoleS for N K G2D ligands in the immune response against human gast ric cancer. Carolina Hager Ribeiro1, Macarena Garrido-Tapia1, Carolina J. Hernández1,2, Karina Kramm1, Marco Bustamante3,

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Juan Carlos Aguillón4,5, and M a ría C a rmen Molina 1,4. 1Programa Disciplinario de Inmunología (ICBM), 2 Escuela de Tecnología Médica, 3Departamento de Cirugía Digestiva, Hospital Salvador, 4Centro de InmunoBioTecnología (ICBM), 5Instituto Milenio de Inmunología e Inmunoterapia – Facultad de Medicina, Universidad de Chile, Santiago, Chile. (86) T he dependence receptor function is conserved by Drosophila F razzled. 1,2 L uis Solano R., 2 Diego Rojas B., 2Álvaro Glavic M. and 1Verónica Palma A. 1Laboratory of Stem Cells and Development. 2 Laboratory of Developmental Biology Faculty of Sciences, University of Chile. FONDAP Center of Genome Regulation. [email protected] (88) T ransactivation of I R E1 and PE R K by a small molecule kinase inhibitor. Morales M 1,2 , Alfaro J1, Mendez A3, Li H3, Gotthardt K3, Walter P3, Shokat K3 and Bernales S1. 1Fundación Ciencia &Vida, 2 Universidad Andrés Bello, 3UCSF. [email protected] (90) Association of N M D A receptor and D L G proteins in the la rval neu romuscula r synapse (N M J) of Drosophila . G uillermo Moya and Jimena Sierralta. Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile. [email protected] (92) M yoblasts fusion is induced by a mechanism involving the vitamin C t ransporter SV C T2 and W nt3a-dependent signaling. Jorge O jeda, Katterine Salazar, Giancarlo V. De Ferrari(2), Francisco Nualart, Juan Pablo Henríquez*. Department of Cell Biology and CMA Bio-Bio, Universidad de Concepcion, Concepcion; (2)Center for Biomedical Research, Universidad Andrés Bello, Santiago; Chile. *[email protected] (94) Involvement of the unfolded p rotein response in locomotor recovery after sciatic nerve in jury. O ñate M. 1,2,3, Hetz C.1,2,4 and Court F.A.3,4. 1Biomedical Neuroscience Institute, 2Center for Molecular Studies of the Cell, University of Chile. 3Millennium Nucleus in Regenerative Biology, Catholic University of Chile. 4Neurounion Biomedical Foundation. [email protected], [email protected], [email protected] (96) T G F-β  induces  neuronal  pola rity th rough a E R K M A P K -mechanism. Osorio J. 1,2, von Bernhardi R.2 and Alvarez A.R.1,3. 1Laboratorio de Señalización Celular, 2Laboratorio de Neurociencia, 3 CARE-Chile-UC, Pontificia Universidad Católica de Chile. [email protected] (98) Neural stem cell differentiation induces a short form of SV C T2. K arina O yarce, Luciano Ferrada and Francisco Nualart. Cell Biology Department, Neurobiology and Stem Cell Laboratory, University of Concepción. [email protected] (100) Sonic H edgehog transactivates and induces not-degradative endocytosis of E G F R in H e L a cells. M argarita Pa rada 1, 2 , Claudia Oyanadel2, Alfonso Gonzalez2 and Verónica Palma1. 1Laboratorio de Células Troncales y Biología del Desarrollo, Facultad de Ciencias, Universidad de Chile. 2Departamento de Inmunología Clínica y Reumatología, Fac. Medicina. Centro de Envejecimiento y Regeneración (CARE). Fac. Ciencias Biológicas. Pontificia Universidad Católica de Chile. [email protected] (102) W nt signaling activation is decreased in a model of motor disease in spite of conserved exp ression of W nt effectors. C ristina Pinto, Nelson Osses(2), Juan Pablo Henríquez*. Department of Cell Biology and CMA Bio-Bio, University of Concepcion, and (2)Institute of Chemistry, P. Catholic University of Valparaiso, Chile. *[email protected] (104) T he antiviral d rug A kt-I V reveals a novel A kt/P E R K pathway that promotes cell death. Daniela Pérez-M unizaga 1, Jennifer Alfaro1, Sebastián Bernales1. 1Fundación Ciencia para la Vida. [email protected] (106) Inhibition of dopamine transporter by a rachidonic acid oxidation is associated with isoketal adduct formation in PC12 cells. 1 Pino J A , 2Torres GE, 1Osses N, 1Reyes JG. 1Instituto de Química, P. Universidad Católica de Valparaíso, Chile; 2Department of Neurobiology, University of Pittsburg, USA. [email protected]

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(108) Differential susceptibility to H 2 O 2-induced death of human lymphocytes cor relates with the reported inverse association between cancer and A lzheimer disease. 1 Ponce DP, 1Silva M, 1Salech F, 1 Merino D, 1Sinning M, 1Torres V, 3Henríquez M, Roe CM, 2,3Quest AFG, 1Behrens MI. 1HCUCH, 2 CEMC, 3ICBM, Faculty of Medicine, UCH [email protected] (110) Role of D L G in the regulation of volume and area of glutamate receptors in the Drosophila la rval neu romuscula r synapse (N M J). M auricio Ramí rez, César Astorga, Jimena Sierralta. BNI, Faculty of Medicine, Universidad de Chile. [email protected] (112) C hanges in localization of C aveolin-1 in acini of saliva ry glands of Sjögren´s Syndrome patients. Díaz-V aldivia N, Bahamondes V, Castro I, Aguilera S, Brito M, Leyton L, Barrera MJ, Quest, A.F.G, González MJ. ICBM, Facultad de Medicina, Universidad de Chile. [email protected] (114) Role of innate immunity during mechanosensory cell regeneration in zeb rafish la rvae. Simón Carrillo, Nicole Reynaert, Miguel L. Allende. FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile. [email protected]; [email protected] (116) Wnt pathways modulate the -7 n A ChR activities in hippocampal neurons and protect neurons from A oligomers. Juan A Godoy1,2, Juvenal A . Rios2 , Felipe G. Serrano1,2, Rodrigo Varas3, and Nibaldo C. Inestrosa1,2. 1Centro de Envejecimiento y Regeneración (CARE), 2Departamento de Biología Celular y Molecular, 3Departamento de Fisiología, Pontificia Universidad Católica de Chile. (118) Rough surface scaffolds with hydroxyl groups enhance osteoblastic differentiation in mesenchymal cells. E dua rdo Roa 1, Juan Reyes1, Juan Pablo Henríquez2 and Nelson Osses1. 1Institute of Chemistry, P. Catholic University of Valparaiso and 2Department of Cell Biology and CMA Bio-Bio, University of Concepción, Chile. [email protected] (120) L ysine-methylt ransferases and demethylases regulate t ranscription of the Runx2 gene during osteogenesis. A d riana Rojas1,2,3, Miguel Allende2 and Martín Montecino1. FONDAP Center for Genome Regulation, 1Faculty of Biological Sciences, Andrés Bello Univesity and 2Faculty of Sciences, University of Chile, 3Faculty of Medicine, Javeriana University. (122) Roles and functional interactions of Drosophila melanogaster K ae1, a putative tR N A modifying enzyme. Rojas-Benítez D. and Glavic A. Centro FONDAP de Regulación del Genoma. Facultad de Ciencias – Universidad de Chile. [email protected] (124) Participation of A D A M metallop roteinases in soluble toll-li ke receptor 2 (s T L R2) p roduction. Rubio E., Langjahr P., Hermoso MA. Innate Immunity Lab, Immunology Program, ICBM, Faculty of Medicine, Universidad de Chile. [email protected] (126) A nti-tumoral function of antigen-specific T h17 cells. Juan José Sáez1, Sarah Núñez1, Felipe Flores1, Mario Rosemblatt1,2,3, María Rosa Bono1, Daniela Sauma1,2. 1Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Fundación Ciencia & Vida. 3Universidad Andrés Bello. [email protected] (128) Identification of molecula r requirements for the ethanol insensitivity in the α3-containing subunit inhibitory glycine receptor. Sánchez. A , Yevenes, G., San Martin. L., Burgos, F., Ramírez, A., Aguayo. L.G. Department of Physiology, University of Concepcion, Chile. [email protected] (130) Identification of LSD1 and associated complexes in the ea rly development of the cerebral cortex. Saud K , Fuentes P, Berndt, F, Kukuljan M. Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile. [email protected]. (132) Bacterial R N As as determinants of human disease. A mi r Shma ryahu and Pablo DT Valenzuela. Fundación Ciencia para la Vida, Av. Zañartu 1482. Ñuñoa. Santiago. Chile. [email protected]

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(134) Regulation of ext racellula r mat rix-associated proteins (E M A Ps) upon Neisseria gonorrhoeae challenge in the F allopian tube. Paula Sola r 1,2, Paula Rodas1,2, Natalia Juica1,2, Luis Velasquez1, 2. 1 Center of Integrative Medicine and Innovative Sciences, Facultad de Medicina, Universidad Andres Bello, Santiago. 2Centro para el Desarrollo de la Nanociencia y Nanotecnologia, Universidad de Santiago de Chile, Santiago, Chile. [email protected] (Sponsor: G. Owen). (136) Predicted N ES and N LS motifs affects subcellula r localization of the ionic ch annel T RPM1. Sotelo P, Brauchi S. Universidad Austral de Chile. [email protected]. (138) Andrographolide activates the W nt pathway and modulates the A PP p rocessing possibly by direct inhibiton of GSK3β. C heril T apia-Rojas1,2, Hianara Bustamante3, Patricia V. Burgos3, Nibaldo C. Inestrosa1,2. 1Centro de Envejecimiento y Regeneración (CARE), 2Departamento de Biología Celular y Molecular, Pontificia Universidad Católica de Chile, 3Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. [email protected] (140) Connective T issue G rowth F actor modulates T G F-beta-dependent mamma ry fibroblast differentiation by a non-Smads-dependent mechanism. Toba r N 1 ., Avalos C1., Urra C1. Smith P.C2 and Martínez J1. 1Laboratorio de Biología Celular y Molecular, INTA, Universidad de Chile and 2Carrera de Odontología. Facultad de Medicina. Pontificia Universidad Católica de Chile. [email protected] (142) Ric-8A is requi red for neu ral crest cells migration. G abriela Toro, Jaime Fuentealba, Lester Riquelme, Marion Rodriguez, Juan Olate, Marcela Torrejón. Universidad de Concepción. [email protected] (144) T he oligomerization of G P C Rs is a dynamic phenomenon modulated by the ligand. T rigo C ., Brauchi S., Gonzalez C. Institute of Physiology, Faculty of Medicine, UACH, Chile. [email protected] (146) In vivo T N F--mediated regulation of C dk5 A ctivity during inflammatory pain. E lias Utreras1,2, Pablo Lazcano2, Pablo Rozas2, Monserrat Barrios2, Christian Gonzalez-Billault2, and Ashok Kulkarni1. 1FGS, LCDB, NIDCR, NIH, Bethesda MD, and 2Laboratory of Cellular and Neuronal Dynamics, Department of Biology, Faculty of Science, University of Chile. [email protected] (148) T he transcription factor C R E B is activated by I G F -1 to regulate myostatin exp ression during myoblast differentiation. Rodrigo Zuloaga, Jorge Aedo, Alfredo Molina and Juan A ntonio V aldés. Universidad Andrés Bello, Facultad de Ciencias Biológicas, Laboratorio de Biotecnología Molecular, Santiago, Chile. [email protected] (150) Targeting  the  UPR  transcription  factor  XBP1  protects  against  Huntington’s  disease  in vivo. V idal, R.1,2,3, Zuleta A. 1,2,, Figueroa A. 1,2, , Molina C.1,2, and Hetz, C. 1,2,3. 1Biomedical Neuroscience Institute, 2Center for Molecular Studies of the Cell, University of Chile and 3Neurounion Biomedical Foundation, Santiago, Chile. [email protected] (152) Defining met rics to compa re skeleton estimation methods applied to biological st ructures. Sergio V illa r roel1,4, Felipe Santibañez1, Omar Ramírez1, Mauricio Cerda1, Karina Palma1,3, Nancy Hitschfeld4, Miguel Concha3, Steffen Härtel1. [email protected]. 1SCIAN-Lab, 2Couve-Lab and 3LeoLab at BNI & Faculty of Medicine; 4DCC; University of Chile. (154) Does Trypanosoma cruzi calreticulin p romote the exp ression of antiangiogenic genes in endothelial cells? V ivanco, F., Duaso, L., Sosoniuk, E., Rosas, C., Aguilar, L., Valck, C*., Ferreira, A*. PDI, ICBM, Facultad de Medicina, Universidad de Chile [email protected] (156) M esoderm and E C M are requi red for coordinated convergence movements of the zebrafish neural plate. C laudia Y efi, Cecilia Arriagada, Miguel Miranda and Claudio Araya. Universidad Austral de Chile. [email protected] (Sponsor: P. Burgos).

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(158) Zeb rafish CSR NPs, csrnp1a and csrnp1b, have redundant functions during nervous system development and hematopoiesis. Zúñiga J., Allende M & Glavic A. Centro FONDAP de Regulación del Genoma. Facultad de Ciencias, Universidad de Chile. [email protected] 18:30 – 20:30

O ral Presentations I I Volcanes Room C hair: M artín Montecino and Co-C hair: V erónica C ambiazo C hronic st ress impai rs cortical synaptic plasticity and expression of b rain-derived neu rot rophic factor in rat. Negrón-O yarzo I. #*, Dagnino-Subiabre A*, Muñoz P#. #Centro Interdisciplinario de Neurociencia de Valparaíso, Universidad de Valparaíso, Valparaíso, Chile. *Centro de Neurobiología y Plasticidad del Desarrollo, Universidad de Valparaíso, Valparaíso, Chile. [email protected] A xonal degeneration p roceeds by necroptosis. Bar rientos S. A . 1, Calixto A.1 and Court, F.A.1,2. Catholic University of Chile and 2Neurounion Biomedical Foundation. [email protected], [email protected] 1

Runx2 and osteopontin promove adhesion of osteosa rcoma cells to human pulmona ry endothelial cells. F rancisco V illanueva 1,2, Oscar Vega1,2, Mercedes Lopez1,3, Flavio Salazar-Onfray1,3, Gary Stein4, Andre van Wijnen5, Mario Galindo1,3. 1Millennium Institute on Immunology and Immunotherapy, 2 Programa de Biología Celular y Molecular, 3Programa de Inmunologia, ICBM, Facultad de Medicina, Universidad de Chile. 4Department of Biochemistry, Vermont Cancer Center for Basic and Translational Research, University of Vermont. 5Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic. [email protected] M etformin, at concentrations cor responding to the treatment of diabetes, potentiates th e cytotoxic effects of ca rboplatin in cultures of ova rian cancer. E rices R 1,3,8, Bravo ML1,8, González P1,8, Oliva B1,8 Racordon D1, Garrido M2, Ibañez C2, Kato S2, Brañes J2, Pizarro J4, Barriga M4, Barra A5, Bravo E6, Alonso C6, Bustamante E7, Jimenez P7, Barrena N7,Cuello M2, and Owen G1,8. Facultades de Ciencias Biologicas1 y Medicina2, Pontificia Universidad Católica de Chile, 3Universidad Andrés Bello. Hospitales: 4Sotero del Rio, 5Barros Luco-Trudeau, 6Gustavo Fricke; 7Fundation Arturo Lopez Pérez. 8 Biomedical Research Consortium of Chile. [email protected] G alectin-8 modulates cell proliferation activating the sonic H E D G E H O G pathway. Remziye Doger 1, Claudia Oyanadel1, Elizabeth Riquelme, Yimo Lin3, Breyan Ross3, Gonzalo Mardones3, Andrea Soza1,2, Alfonso Gonzalez1,2. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina1. Centro de Envejecimiento y Regeneración (CARE) Fac. Ciencias Biológicas2. Pontificia Universidad Católica de Chile. Instituto de Fisiología Facultad de Medicina, Centro de Investigación Sur-Austral en Enfermedades del Sistema Nervioso (CISNe), Universidad Austral de Chile, Valdivia3. [email protected] A transcriptomics analysis of spinal cord regeneration in Xenopus laevis. Dasfne Lee-L iu 1 , Mauricio Moreno1, Leonardo I. Almonacid2, Víctor Tapia1, Rosana Muñoz1, Francisco Melo2, Juan Larraín1. 1 Center for Aging and Regeneration and Millenium Nucleus in Regenerative Biology; 2Molecular Bioinformatics Laboratory, Millennium Institute on Immunology and Immunotherapy; Pontificia Universidad Católica de Chile. [email protected] C ellula r st ress enhances tu rnover of C99 dependent on proteasomal and lysosomal activities. A lexis González, Hianara Bustamante, Viviana Cavieres, Vanessa Muñoz, Andrés Rivera-Dictter, Gonzalo A. Mardones and Patricia V. Burgos. Instituto Fisiología, Facultad de Medicina, Universidad Austral Chile, Valdivia Chile. [email protected] M uscle fibe r degeneration induced by A T P stimulation in a dystrophic mouse model. V allada res D., Almarza G., Jaimovich E. Centro de Estudios Moleculares de la Célula, ICBM, Facultad de Medicina, Universidad de Chile. [email protected] 20:30

Dinner

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22:00 – 23:00

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Plena ry Lectu re M illennium Institute on Immunology and Immunotherapy Volcanes Room C hair: A lexis K alergis C hronic Salmonella car riage is controlled by host a host regulator of fatty acid metabolism. Denise Monack. Department of Microbiology and Immunology, Stanford School of Medicine, Stanford University, CA, USA.

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F R I D A Y , O C T O B E R 26, 2012 08:00

Poster Mounting Session I I I: N° 160 to N° 238 Convention C enter Foyer

09:00 – 11:00

Symposium Proyectos F O N D E C Y T de Cooperación Internacional 1120512 (A A) - 1110310 (SZ) C albuco Room C hair: A lejand ra A lva rez and Co-C hair: Silvana Zanlungo “To Die or Not to Die: Neuron Signaling” T riosephosphate isomerase nit roty rosination by amyloid ß-peptide induces neuronal death. Marta Tajes, Fanny Rubio-Moscardó, Biuse Guivernau, Eva Ramos-Fernández, Abel Eraso, Mònica BoschMorató, Ernest Palomer, Francesc-Xavier Guix and F rancisco J. M uñoz. Laboratory of Molecular Physiology and Channelopathies, Universitat Pompeu Fabra (UPF), Barcelona, Spain. [email protected] T he c-A bl/p73 p roapoptotic system is a relevant signaling pathway involved in Niemann-Pick type C neuron death. Silvana Zanlungo1 , Mary Carmen Vázquez1, Andres Klein1, Lina M. Vargas2, Fermín Robledo1, Francisco J. Muñoz3, Alejandra R. Alvarez2. 1Departamento de Gastroenterología, Facultad de Medicina, 2Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile.3 Grup de Fisiologia Molecular i Canalopaties, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain. [email protected] c-A bl activation mediates the A  O induced synapses loss; a new signaling involved in A lzheimer's disease synaptic alterations. A lejand ra R. A lva rez1 , Lina M. Vargas1, Gonzalo I. Cancino1, Nancy R. Leal1, Lisbell A. Estrada1, Elena Pasquale3 , Silvana Zanlungo2. 1Laboratorio de Señalización Celular, Departamento de Biología Celular y Molecular., Facultad de Ciencias Biológicas 2Departamento de Gastroenterología, Facultad de Medicina, Pontificia Universidad Católica de Chile, 3Sanford-Burnham Medical Research Institute, La Jolla, California, USA. A ß oligomers alter zinc neu romodulatory signaling:  Relevance  to  Alzheimer’s  disease.  Jorge Busciglio and Emily Vogler. Department of Neurobiology and Behavior, Institute for Memory Impairments and Neurological Disorders (iMIND), and Center for the Neurobiology of Learning and Memory (CNLM), University of California, Irvine, CA 92697. Symposium A nillo A T C-71 e Instituto M ilenio, C entro Interdiciplinario de Neu rociencias de V alpa raíso. T ronador Room C hair: Juan C arlos Saez “Intercellula r Comunication V ia Pannexin and Connexin-based C hannels in H ealth and Disease” A hemichannel const ruct rescues left-right patterning in emb ryos depleted of Connexin43.4: implications for epithelial lumen formation. Julia M. Hatler1, Jeffrey J. Essner2,  Scott  O’Grady3 and Ross G . Johnson 1. 1Dept. of Genetics, Cell Biology and Development, Univ. of Minnesota, Minneapolis, MN, and 2Dept. of Genetics, Development and Cell Biology, Iowa State Univ., Ames, IA and 3Dept. of Animal Science, Univ. of Minnesota, St. Paul, MN USA. Connexin channels and cell calcium, two partners engaged in extensive cross-talk. L uc L eybaert, Dept. Basic Medical Sciences, Fac. Medicine & Health Sciences, Ghent University, Ghent, Belgium. T he cellula r life of pannexins and their function in health and disease. Silvia Penuela, Dale W. Laird. Department of Anatomy and Cell Biology, University of Western Ontario, London ON N6A5C1 Canada.

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Glial  hemichannels:  an  alternative  target  for  neuroprotection  in  Alzheimer’s  disease. C h ristian G iaume, CIRB, Collège de France, Paris, France. 10:30 – 13:00

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

11:00 – 13:00

Poster V iewing Session I I I: 160-238 O dd Numbers Convention C enter Foyer Coordinators: Pat ricio Smith – C laudia M etz – M aría de los A ngeles G arcía (161) C haracterization of A359D mutation in acid sphingomyelinase causing Niemann-Pick type B disease in chilean patients. A cuña M 1 , Martínez P1, Latorre M2, Castro V3, Lagos M4, Guiche V3, González M3,5, Miquel JF1,5, Mabe P6, Zanlungo S1,5. 1Departamento de Gastroenterología, Facultad de Medicina, Pontificia Universidad Católica de Chile, 2INTA, Universidad de Chile, 3Facultad de Ciencias Universidad de Chile, 4 Departamento de Laboratorios Clínicos, Pontificia Universidad Católica de Chile, 5 FONDAP Center for Genome Regulation (CGR), 6Hospital Exequiel González Cortés. [email protected] (163) Reduced C T G F exp ression p revents fibrosis in the stressed myoca rdium. Riquelme, C A, Morales, G, Godoy, I* and Brandan, E. Center for Aging and Regeneration (CARE), Faculty of Biological Sciences; *Cardiology Laboratory, School of Medicine. Catholic University of Chile. [email protected] (165) A nti-inflammatory effects of chitosan nanoparticles in human gingival fib roblasts. A rancibia R, Silva D, Cáceres M, Tobar N, Tapia C, Martinez J, Smith PC. Carrera de Odontología. Facultad de Medicina. Pontificia Universidad Católica de Chile, Facultad de Ciencias Químicas y Farmacéuticas e INTA, Universidad de Chile. [email protected] (167) C haracterization of a muscle cell line stably exp ressing recombinant Pannexin-1. A rias, M 1, Jaimovich, E1 and Buvinic, S1,2. 1Laboratorio de Fisiología Celular del Músculo, Centro de Estudios Moleculares de la Célula, ICBM, Facultad de Medicina; 2 Departamento de Ciencias Básicas y Comunitarias, Facultad de Odontología. Universidad de Chile. Santiago, Chile. [email protected] (169) Syndecan-4 inhibits Wnt/β-catenin pathway, regulates the levels of signaling components, and is requi red for the inhibitory function of W nt5a. Pablo Astudillo, Nicolás Cumplido, Héctor Carrasco and Juan Larraín. Laboratory of Developmental Biology and Regeneration, Center for Aging and Regeneration, Pontificia Universidad Católica de Chile. [email protected] (171) E xp ression of the human A PE1 endonuclease and its negative dominant form in T. cruzi epimastigotes exposed to oxidative agents. Paula Bahamondes, Iván Ponce, Sofia Sepúlveda, Lucía Valenzuela, Santiago Ramírez, Soledad Sierra, Natalia Muñoz, Ulrike Kemmerling, Norbel Galanti, Gonzalo Cabrera. Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. [email protected] (173) Interplay between st ress hormones and brain monoamines in the insula r cortex modulates the reaction to emotionally a rousing experiences. Rodrigo Moraga-Amaro, Raúl Díaz, Andrés Cortes, Sebastián Rojas, Jimmy Stehberg. Universidad Andrés Bello. [email protected] (175) C haracterization and response to chemotherapy of cancer cell populations originating from ovarian cancer patients. 1,8 B ravo, M L., 1,8Gonzalez, P., 1,8Kato, S., 2Brañes, J., 2Garrido, M., 2Ibañez, C., 3 Bravo, E., 3Alonso, C., 4Jimenez, P., 4Bustamante, E., 4Barrena, N., 5Barra, A., 6Celis, M., 6Aguilera, B., 7 Barriga, MI., 7Pizarro, J., 2,8Cuello, M., 1,8Owen, GI. 1Factultad de Ciencias Biologicas y 2Medicina, Pontificia Universidad Católica de Chile. Hospitals: 3Gustavo Fricke, 4Fundación Arturo López-Pérez, 5 Barros Luco, 6 Luis Tisné, 7Sótero del Río. 8Biomedical Research Consortium of Chile (CTU06). [email protected] (177) Identification of common genetic variants between A lzheimer's disease (A D) and Schizoph renia (SZ). Bernabé Bustos, Camilo Villamán, Eduardo Pérez, Mario Caracci, Flavia Román,

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Pablo León and Giancarlo V. De Ferrari. Center for Biomedical Research, Universidad Andrés Bello, Santiago, Chile. [email protected] (179) PSD-95 suppresses dend ritic a rbor development in matu re hippocampal neu rons by occluding synaptic insertion of N R2B-N M D A receptors. M atias C ampos1, Fernando Bustos1,2, Lorena VarelaNallar3, David Gonzalez1, Berta Henriquez1, Nibaldo Inestrosa3 and Brigitte van Zundert1*. 1Andres Bello 2 3 University; University of Concepción; Pontifical Catholic University de Chile. [email protected] (181) E PC cells as a model of ribosomal biogenesis in cyprinus ca rpio during seasonal acclimatization. Fumeron R., C a rvajal PA.*, Rudolffi P., Dupre G., Molina A., Vera MI., Alvarez M. Laboratorio de Biología Celular y Molecular, F. de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile, *Biotecnología, Universidad de Antofagasta. [email protected] (Sponsor: G. Arriagada). (183) Controlled heterodimerization of C99 with organelle resident proteins p rovide information -secretase. V iviana C avieres, Andrés Rivera-Dictter, Prabbuddha Sengupta, Gonzalo Mardones and Patricia V. Burgos. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. [email protected] (185) Role  of  IRE1α  in  cytoskeleton  regulation  and  cell  migration:  a  novel  function  beyond  ER  stress. U r ra H 1,2 ., Rodríguez R1,2., Henríquez D3., González-Billault C3., and Hetz C1,2. 1Biomedical Neuroscience Institute¸ 2Center for Molecular Studies of the Cell, Faculty of Medicine, 3Department of Cell Biology, Faculty of Sciences, University of Chile. Santiago, Chile. [email protected] (187) C dk5 regulates Rap1 activity through phosphorylation of C3G . Elias Utreras1, E rick Contreras-V allejos1, Daniel Henriquez1, Cristina Olmos1, Alex Di Genova2, Alejandro Maass2, Ashok Kulkarni3 and Christian Gonzalez-Billault1. 1Laboratory of Cell and Neuronal Dynamics, Department of Biology, Faculty of Sciences and 2Mathematical Modeling Center, Faculty of Physics and Mathematics Sciences, Universidad de Chile and 3LCDB, NIDCR, NIH, USA. [email protected] (189) A ntidepressant effect on functional recovery and neurot rophic factors exp ression in an animal model of int racortical stroke. *V alentina Contreras, *Rodrigo Zepeda, *Claudia Pissani, Gareth I. Owen, Francisca C Bronfman. Physiology Department, Pontificia Universidad Católica de Chile. *Contributed equally. [email protected], [email protected] (191) Development of an R N A i-based genetic adjuvant silencing ST A T3 for D N A vaccines against tumors. Nicole Rojas-Colonelli, Andrés Herrada, Paola Murgas, Manuel Varas, Alvaro Lladser. Laboratory of Gene Immunotherapy, Fundación Ciencia & Vida. [email protected] (193) Constitutive endocytosis of the sodium-dependent vitamin C transporter 2 SV C T2. 1 A driana Covar rubias, 1Aníbal I. Acuña, 2Sebastian Brauchi, and 1Maite A. Castro. 1Instituto de Bioquímica y Microbiología, 2Instituto de Fisiología, UACh. [email protected] (195) Angiotensin  II  induces  fibrosis  in  kidney  tubule  epithelial  cells  via  Wnt/β-catenin signaling pathway. C atherina A . C uevas1,2,4, Alexis A. González4,5, Nibaldo C Inestrosa1,3 , Carlos P. Vio1,2 , Minolfa C Prieto3,4. 1Center for Aging and Regeneration, 2Department of Physiology, 3Department of Cellular and Molecular Biology, P. Universidad Católica de Chile, Santiago, Chile. 4Department of Physiology, 5Hypertension and Renal Center of Excellence, Tulane University, New Orleans, LA, USA. [email protected] (197) E xt racellula r A T P p roduces both H 2 O 2 increase and NR F 2 activation in cultured fibers of skeletal muscle. Díaz A .1,2, Campos C.1, Espinosa A1 and Jaimovich E2. 1Escuela de Tecnología Médica, Universidad de Chile and 2Centro de Estudios Moleculares de la Célula, Facultad de Medicina, Universidad de Chile. [email protected] (199) New elements of W nt-5a signaling in hippocampal neu rons: Identification of frizzled receptor and role of G p rotein. V . T. Ramirez and N.C. Inestrosa. Centro de Envejecimiento y Regeneración

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(CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. [email protected] (201) Discovery and assessment of novel Dpp/M ad target genes and their enhancers in the early D. melanogaster emb ryo. C alixto Domínguez, Michael Pino, Rodrigo Pulgar and Verónica Cambiazo. Laboratorio Bioinformatica y Expresión Génica, INTA-Universidad de Chile and Fondap Center for Genome Regulation (CRG). [email protected] (203) A novel function of the ER stress sensor IRE1α in DNA damage responses.  Estefanie Dufey 1,2, Diego Rodriguez1,2, Diego Rojas-Rivera1,2, Hery Urra1,2, Juana Pincheira3 and Claudio Hetz1,2,4. 1 Biomedical Neuroscience Institute, 2Center for Molecular Studies of the Cell, 3Human Genetics Program, Faculty of medicine, and 4Neurounion Biomedical Foundation, Santiago, Chile. [email protected] (205) L actate affects the exp ression of orexigenic and anorexigenic neu ropeptides in hypothalamic neurons. E lizondo-V ega R, Carril C, Cortés-Campos C, Nualart F, García-Robles M. Departamento de Biología Celular, Universidad de Concepción. [email protected] (207) Recombinant W nt3 distributes in a vesicula r pattern in chick motor neu rons and motor neuron-li ke NSC-34 cells. Javier Espinoza, Pedro Zamorano(2), Juan Pablo Henríquez*. Department of Cell Biology and CMA Bio-Bio, University of Concepción, and (2)Faculty of Health Sciences, University of Antofagasta, Chile. *[email protected] (209) E valuation of the epigenetic response generated by E N OSC complex during cellula r energy rest riction in Cyprinus carpio seasonal acclimatization. Fernández de la Reguera C ., Nardocci G., Carvajal P., Molina A., Vera MI., Alvarez M., L. de Biología Celular y Molecular, F. de Ciencias Biológicas, U. Andrés Bello, Viña del Mar, Chile. [email protected]; [email protected] (211) Skeletal muscle hypert rophy in fish in vivo is regulated via A kt/T O R and M A P K /E R K signaling pathways affecting differentially the I G F system and atrogenes. E duardo N. F uentesa, Ingibjörg Eir Einarsdottirb, Rodolfo Paredesa, Christian Hidalgoa, Juan Antonio Valdesa, Björn Thrandur Björnssonb, Alfredo Molinaa. aFacultad de Ciencias Biológicas, Universidad Andrés Bello, Av. Republica 217, Santiago, Chile. bFish Endocrinology Laboratory, University of Gothenburg, Box 463, S-40530 Göteborg, Sweden. (213) Potential biological ma r kers in melanoma patients treated with immunotherapy based in dendritic cells. G arcia T, Tittarelli A, Villablanca A, Pereda C, Matthäus F, Beltrán A, López M, Norgauer J, Hoheisel J, Gebicke-Haerter P, Salazar F. Tumor Immunology Laboratory, Faculty of Medicine, University of Chile. [email protected] (215) Comparative exp ression of P C N A , A M A C R and H M W C K in human, canine and experimental mu rine p rostatic lesions, and their potential diagnostic value. González C 1; Armisén R2; Stutzin A2; Landerer E1; Paredes R1; Contreras I1; Muñoz C1. 1Universidad Andrés Bello, 2Centro de Estudios Moleculares de la Célula & Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. [email protected] (217) Personalized cancer therapy for ova rian cancer patients. P. González1,8, M.L. Bravo1,8, S. Kato2,8, M. Garrido2, J. Brañes2, J. Pizarro3, M.I. Barriga3, H. Leon4, E. Bustamante4, C. Alonso5, E.Bravo5, M .A. Celis6, B. Aguilera6, A. Barra7, N. Barrena4, C. Ibañez4,P. Jimenez4, M.A Cuello2,5, G. I. Owen1,5. 1Facultad de Ciencias Biológicas, 2Facultad de Medicina, Pontificia Universidad Católica de Chile, Hospitales: 3Sotero del Rio, 4Fundación Arturo López Pérez, 5Gustavo Fricke, 6Luis Tisné, 7Barros Luco. 8Biomedical Research Consortium of Chile (CTU06) & FONDECYT 3120003. [email protected] (219) A n inducible novel apical-basolateral transcytotic route in polarized epithelial cells. C atalina G rabowski 2, Claudio Retamal2, Fabiola Sánchez3 and Alfonso González1,2. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina1. Centro de Envejecimiento y Regeneración (CARE). Fac. Cs. Biológicas2. Pontificia Universidad Católica de Chile. Departamento de Inmunología, Facultad de Medicina, Universidad Austral de Chile3. cgrabowskipgmail.com

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(221) Identification and characterization of lymphoid T cells of atlantic salmon ( Salmo salar) using monoclonal antibodies. H alca rtéga ray N., Wilhelm V., Bono M.R., Valenzuela P., Rosemblatt M. Fundación Ciencia y Vida, Universidad Andrés Bello, Sciences Faculty, Universidad de Chile. [email protected] (223) A ltered N F-–dependent gene expression after depola rization-induced R OS in dystrophic M D X muscle cells. H enríquez C ., Altamirano F. and Jaimovich E. Centro de Estudios Moleculares de la Célula, ICBM, Facultad de Medicina, Universidad de Chile. Santiago, Chile. (225) T he two major regulators of ext ra-emb ryonic tissue specification are conserved in higher dipterans outside of D rosophila species. C h ristian Hodar Q ., Verónica Cambiazo A. Laboratorio Bioinformatica y Expresión Génica, INTA-Universidad de Chile and Fondap Center for Genome Regulation (CRG). [email protected] (227) E ffects of H M G-CoA reductase inhibition in craniofacial development of zeb rafish. C arolina Jeréz1, Allison Ávalos1, Karol Cortés1, José Suazo2 and Alicia Colombo1. 1Institute of Biomedical Sciences, University of Chile, Santiago, Chile; 2School of Medicine, P. Universidad Católica de Chile. [email protected] (229) E xtracellula r mat rix-associated proteins exp ression in F allopian tubes explants infected with Neisseria gonorroheae . Natalia Juica1,2 , Paula Solar1, Luis Velásquez1. 1Center for Integrative Medicine 2 and Innovative Science, Universidad Andrés Bello Universidad de Santiago de Chile. [email protected] (Sponsor: R. Moreno). (231) T iam1/Rac1 are requi red for D I T N C1 astrocyte migration induced by T hy-1. K ong M, Cárdenas A, Alvarez A, Quest AFG, Leyton L. Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula. Facultad de Medicina. Universidad de Chile. [email protected] (233) Identification of H N T targets in Drosophila photoreceptor cells. Estefanía López, Carlos Oliva, Patricio Olguín, and Jimena Sierralta. BNI, ICBM, Facultad de Medicina, Universidad de Chile. VIB Center for the biology of disease, Katholieke Universiteit Leuven. [email protected] (235) Astrocyte αvβ3-integrin induces neuronal ret raction by increasing T hy-1-C BP interaction and Src inactivation. M aldonado, H ., Ramirez, O., Haertel, S., Quest, A.F.G., Leyton, L. Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula. Facultad de Medicina. Universidad de Chile. [email protected] (237) RPR M gene silencing by methylation in gastric cancer models. M archant M .1,2 , Guzman L.1 and Corvalán A.2. 1Instituto de Química, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Valparaíso. 2Centro de Investigaciones Médicas, Pontificia Universidad Católica de Chile. [email protected] 13:00 – 15:00

L unch

15:00 – 17:00

O ral Presentations I I I Volcanes Room C hair: F rancisco Nuala rt and Co-C hair: M aite C astro T he Rab11-recycling endosome is required for B D N F-induced activation of C R E B transcription factor in hippocampal neurons. A ndrés González, Oscar Lazo, and Francisca Bronfman. Neuronal Cell Biology and Regeneration Lab, Pontificia Universidad Católica de Chile. [email protected] Delivery of A deno Associated V i ruses to exp ress X BP-1s in central nervous system p rolongs life span of an A LS mouse model. V alenzuela V . 1,2,3, Matus S.1,2,3, Nasiff M.1,2,3 and Hetz C.1,2,3. 1 Biomedical Neuroscience Institute, Faculty of Medicine. 2Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile, Santiago, Chile. 3Neurounion Biomedical Foundation, Santiago, Chile.

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Scorbutogenic diet reduces angiogenic activity in a guinea pig model for glioblastoma multiforme. Rodríguez, F ., Jara, N., Salazar, K. and Nualart, F. Neurobiology and Stem Cells Laboratory, University of Concepción. [email protected] Induced alloantigen-specific regulatory T cells generate mixed chimerism and allograft tolerance. Paula M aldonado1, Paulina Ruiz1, Yessia Hidalgo1, Mario Rosemblatt1,2,3, María Rosa Bono1. 1 Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2Fundación Ciencia & Vida. 3 Universidad Andrés Bello. [email protected] H istone Modifications and D N A methylation, key regulators of Osterix gene exp ression. H ugo Sepúlveda, Candy Rojas and Martín Montecino. Center for Biomedical Research and FONDAP Center for Genome Regulation, Faculty of Biological Sciences and Faculty of Medicine, Andres Bello University. T he c-A bl tyrosine kinase phosphorylates H D A C2 and increases its activity and protein levels: a new mechanism of regulation of the gene expression. González Z. M . 1,2, Contreras P.1,2, Chamorro D.1,2, Estrada L.1,2, Seto-E.3, Villagra A.3, Álvarez A.R.1,2. 1Laboratorio de Señalización Celular, 2CAREChile-UC, Pontificia Universidad Católica de Chile. 3H..Lee.Moffitt Cancer.Center and Research Institute,.USA. [email protected] S-nitrosation of P120 and V ASP: a new mechanism in the regulation of microvascula r permeability. 1 Zamorano P, 1Marín N, 1Burboa P, 2Boric M, 3Durán WN, 1Sánchez FA.1 Instituto de Inmunología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. 2Departamento de Ciencias Fisiológicas, Pontificia Universidad Católica de Chile. 3Department of Pharmacology and Physiology, New Jersey Medical School, Newark, New Jersey, USA. [email protected] (Sponsor: C. Figueroa). Detection of flap endonuclease activity in Trypanosoma cruzi. Iván Ponce, Sofía Sepúlveda, Lucía Valenzuela, Santiago Ramírez, Soledad Sierra, Paula Bahamondes, Gilda Garrido, Natalia Muñoz, Ulrike Kemmerling, Gonzalo Cabrera, Norbel Galanti. Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. [email protected] 16:30 – 19:00

Schools and Science: Students from Puerto V a ras at the C hilean Society for C ell Biology Room Business C enter

17:00 – 19:00

Poster V iewing Session I I I: 160-238 E ven Numbers Convention C enter Foyer Coordinators: Pat ricia Burgos – Gonzalo M ardones – C arlos B. González (160) Rab5 activation promotes cell migration and focal adhesion turnover. Pablo M endoza 1,2, Jorge Díaz2, Rina Ortiz2, Andrew F.G Quest2, Lisette Leyton2, Dwayne Stupack3 and Vicente Torres1,2. 1 Laboratory of Celular and Molecular Biology, Faculty of Dentistry, Universidad de Chile. 2Laboratory of Cellular Comunications, FONDAP Centre for Molecular Studies of the Cell, Faculty of Medicine, Universidad de Chile. 3Moores UCSD Cancer Center, University of California San Diego, USA. [email protected] (162) In vivo single cell labeling by focal elect roporation on the b rain of zeb rafish la rvae. M eynard M .M., Armijo L., Guerrero N., Palma K. and Concha M.L. Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile. [email protected] (164) Ascorbic  acid  metabolic  switch  failure  in  Huntington’s  disease.  1 Pablo Moll, 1Felipe Beltrán, 1 Aníbal I. Acuña, 2Claudio Hetz, 1Ilona I. Concha, 3Michael Levine, 1Maite A. Castro. 1Instituto Bioquímica y Microbiología, UACh; 2ICBM, U. Chile 3Semel Institute, UCLA. [email protected] (166) T he role of p rotein S-nitrosylation in neurotoxocity versus neu rop rotection. Barba ra M aturana, Ariel Caviedes, Felipe Gordillo, Veronica Martinez, Thilo Kaehne, Ursula Wyneken. Universidad de los Andes, Otto-von-Guericke University. [email protected]

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(168) V asopressin induces m T O R activation in vascula r smooth muscle cells cultu red in high ext racellula r glucose concent ration. Daniela K . Montes, Vanessa C. Muñoz, Patricia V. Burgos, Carlos B. González. Instituto de Fisiología. Facultad de Medicina. Universidad Austral de Chile. [email protected] (170) Tolerogenic dend ritic cells acquire a specific migrato ry pattern when activated with monophosphoryl lipid A. Rod rigo Morales, Lorena Hoyos, Paulina García, Karina Pino-Lagos, Bárbara Pesce, Diego Catalán, Juan Carlos Aguillón. Immune Regulation and Tolerance Group (www.irtgroup.cl), Programa Disciplinario de Inmunología, Instituto de Ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile. [email protected] (172) C99 is degraded by autophagy in an ubiquitin-dependent manner. V anessa M uñoz, Hianara Bustamante, Andrés Rivera-Dictter, Alexis Gonzalez, Viviana Cavieres, Gonzalo A. Mardones y Patricia V. Burgos. Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile. [email protected] (174) Drosophila phosphatidate phosphatase-2 (dPA P2) modulates bina ry cell fate decision mediated by Notch signaling. Patricio O lguín, Sophie Balmer, Ursula Weber and Marek Mlodzik. Programa de Genética Humana, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Mount Sinai School of Medicine, New York, NY, USA. [email protected] (176) E pigenetic characterization of Runx2 gene P1 and P2 promoters: Identification of potential new regulatory elements. T atiana O pazo, Hugo Sepúlveda and Martín Montecino. Center for Biomedical Research and FONDAP Center for Genome Regulation, Faculty of Biological Sciences and Faculty of Medicine, Universidad Andrés Bello. (178) G alectin-8 induces partial epithelial-mesenchymal transition in M D C K cells. C laudia O yanadel, Evelyn Pardo, Remziye Doger, Marcela Bravo-Zehnder, Andrea Soza, and Alfonso González. Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas. Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina. Pontificia Universidad Católica de Chile. (180) Dopamine receptor D3 exp ressed on C D4+ T-cells favou rs neu rodegeneration of dopaminergic neu rons du ring Pa r kinson's disease. Hugo Gonzalez1, Francisco Contreras1, Carolina Prado1, Daniela Elgueta1 Dafne Franz1 and Rodrigo Pacheco1,2 . 1Fundación Ciencia & Vida and 2 Universidad San Sebastián. Santiago, Chile. [email protected] (182) Proteomic analysis of germinal layer from hydatid cyst of Echinococcus granulosus. Christian Hidalgo and Rodolfo Pa redes. Escuela de Medicina Veterinaria, Facultad de Ecología y Recursos Naturales, Universidad Andrés Bello. [email protected] (184) SD F1a as a retention signal for zeb rafish neutrophils during inflammation resolution. Osca r A . Peña, Carlos Rubilar, Miguel L. Allende. FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile. [email protected]; [email protected] (186) C haracterization of the motor phenotype of p75 N T R null mice. V iviana Pérez(1), Andrés González(2), Francisca Bronfman(2), Juan Pablo Henríquez(1)*. (1)Department of Cell Biology and CMA Bio-Bio, University of Concepcion, and (2)Department of Physiology, P. Catholic University of Chile. *[email protected] (188) Assessment of the exp ression of a chimeric G rowth Hormone ( Seriola sp.) and their recruitment to the secretory pathway. M arysol Paz, Juan Carlos Leiva, Pedro Zamorano*. Faculty of Health Sciences, University of Antofagasta, Chile. *[email protected] (190) H uman platelet factor X I I I: synthesis and functional role in hemostasis. Pozo L. M ., Panes O., Matus V., Sáez CG, Pereira J., Mezzano D. Dpto. Hematología-Oncología, Escuela de Medicina, Pontificia Universidad Católica de Chile. [email protected]

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(192) E ndoplasmic reticulum organization and modulation of calcium signals in ast rocytes. O ma r Ramí rez1,2,3, Alex Córdova3, Jorge Toledo1,2,3, Mauricio Cerda1,2,3, Andrés Couve1,2, Steffen Härtel1,2,3. 1 Program of Anatomy and Developmental Biology, 2Biomedical Neuroscience Institute (BNI), 3SCIANLab, Faculty of Medicine, University of Chile, Santiago, Chile. [email protected] (194) Role of I K D current in hypersensitivity to cold induced by peripheral injury in prima ry sensory neu rons. Rest repo, C . 1, Piña, R.1,2, Ugarte, G. 1, Bacigalupo, J.2, Pertusa, M. 1 and Madrid, R.1. 1 Facultad de Química y Biología, Universidad de Santiago de Chile; 2Facultad de Ciencias, Universidad de Chile. [email protected] (196) E valuation of inflammatory cytokines p rofile in the physiopathology of p reeclampsia. Ribeiro A ., Tempio F., Gatica J., Falcón C., Falcón F., Behrens C., Guerrero P., Pardo A., Parra C., López M.N. Program of Immunology, ICBM, Faculty of Medicine, Clinical Hospital University of Chile. [email protected] (Sponsor: F Salazar). (198) Neuropeptide secretion during aging in C. elegans. Alicia N. Minniti2 and Rebeca A ldunate1,2 . 1 Escuela de Biotecnología, Universidad Santo Tomás; 2Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. [email protected] (200) A blation of the gallbladder decreases basal metabolic rate and favors fatty liver disease in mice. Fermín Robledo1, Ludwig Amigo1, Javiera Gallardo1, Kurt Schalper2, Víctor Cortés3, Daniel Bastías4, Silvana Zanlungo1, Juan Francisco Miquel1, Sergio González2, Marco Arrese1, Francisco Bozinovic4, Flavio Nervi1. 1Departamentos de Gastroenterología, 2Anatomía Patológica, 3Nutrición y Diabetes, Facultad de Medicina, 4CASEB and Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. [email protected] (202) A LS astrocytes release factors that trigger motoneuron death by increasing sodium channel activity. F abiola Rojas1,2, Elsa Fritz1,2, David Gonzalez2, Sebastian Abarzua2, Nicole Cortes2, Brigitte van Zundert2. 1Universidad de Concepción; 2Universidad Andrés Bello. [email protected] (204) Indirect t ranscytotic apical sorting of Sonic H edgehog in pola rized M D C K cells. Lorena Rosa1,2 , Mariana Labarca1,2, Claudio Retamal1,2, Juan Larraín2 and Alfonso González 1,2. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina1. Centro de Envejecimiento y Regeneración (CARE). Fac. Cs. Biológicas2. Pontificia Universidad Católica de Chile. [email protected] (206) M ild copper overload modulates energetic output and induces O X PH OS supercomplexes remodeling in the eryth ropoietic cell line K 562. L ina Ruiz, Erik Jensen, Alan Briones and Alvaro Elorza Experimental Bioenergetics Laboratory, Universidad Andres Bello (UNAB). [email protected] (208) T he role of K L O T H O in the regulation of adult hippocampal neu rogenesis. Felipe Salech 1,2,3, Lorena Varela-Nallar4, Magdalena Gonzalez1, Luis Michea1, Nibaldo C. Inestrosa4, María Isabel Behrens1,3 and Andrés Couve1,2. 1Physiology and Biophysics, ICBM, 2Biomedical Neuroscience Institute (BNI), Facultad de Medicina, Universidad de Chile, Santiago, Chile. 3Hospital Clínico Universidad de Chile, Santiago, Chile. 4Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. [email protected] (210) A nalysis of colocalization with super resolution optical fluctuation imaging (SO F I). Felipe Santibáñez-Leal1, Omar Ramírez1, Anja Huss3, Jörg Enderlein3, Andrés Couve2 & Steffen Härtel1. 1 SCIAN-Lab and 2Couve-Lab at BNI, ICBM, Faculty of Medicine, U. of Chile. 3III. Physikalisches Institut, Universität Göttingen, Germany. [email protected] (212) E xpression and localization of components of the I R E-1Α/XBP-1 pathway of the misfolded protein response in saliva ry glands from Sjögren's Syndrome patients. Sepúlveda D, Aguilera S, Bahamondes V, Barrera MJ, Castro I, Molina C, González S, Urzúa U, Leyton C and González MJ. ICBM-Facultad de Medicina, Universidad de Chile. [email protected].

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(214) A purinic/apyrimidinic Tc A P1 endonuclease and Trypanosoma cruzi resistance to persistent oxidative stress. Soledad Sier ra, Sofia Sepúlveda, Lucía Valenzuela, José Delgadillo, Santiago Ramírez, Iván Ponce, Paula Bahamondes, Natalia Muñoz, Ulrike Kemmerling, Norbel Galanti, Gonzalo Cabrera. Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile. [email protected] (216) Monocarboxylates a re not important to support synaptic activity  in  Huntington’s  disease.  M acarena Solís, 1Felipe Beltrán, 1Aníbal I. Acuña, 1Alejandra Parra, 2Claudio Hetz, 1Ilona I. Concha, 3 Michael Levine, 1Maite A. Castro. 1Instituto Bioquímica y Microbiología, UACh; 2ICBM, U. Chile 3 Semel Institute, UCLA. [email protected] 1

(218) E ph A7 exp ression during posterior commissu re development. Stanic K , Vera A, Montecinos H, Caprile T. Departamento Biología Celular, Universidad Concepción, Chile. [email protected] (220) Immune genes exp ression in response to spinal cord injury in Xenopus laevis. V íctor T apia 1, Mauricio Moreno1, Dasfne Lee1, Leonardo Almonacid2, Francisco Melo2, Juan Larraín1. 1Center for Aging and Regeneration and Millennium Nucleus in Regenerative Biology; 2Molecular Bioinformatics Laboratory, Millennium Institute on Immunology and Immunotherapy; Pontificia Universidad Católica de Chile. [email protected] (222) T he organization of the tubular networ k of the endoplasmic reticulum modifies the tran sport of ca rgo p roteins. Jorge Toledo1,2,3, Luis Briones2,3, Mauricio Cerda2,3, Jorge Jara2,3, Steffen Härtel2,3, Omar Ramírez2,3,and Andrés Couve1,2. 1Program of Physiology and Biophysics, 2Biomedical Neuroscience Institute (BNI), 3SCIAN-Lab; ICBM, Facultad de Medicina, Universidad de Chile. [email protected] (224) The  M105I  mutation  of  αSNAP  reduces  its  brain  membrane  binding  properties.  G abriela Toro1, Camilo Muñoz1, Guillermo Márquez1, Breyan Ross2, Gonzalo Mardones2, Luis Federico Bátiz1. Institutos de Histología1 y Fisiología2, Facultad de Medicina. Universidad Austral de Chile. [email protected] (226) Selective inhibition of protein t ranslocation into the E R as a novel t reatment for multiple myeloma. U reta, G . 1,2, Amoroso, A.3, McCullagh, E.1, Maglathlin, R.4, Taunton, J.4, Bernales, S1. 1 Fundación Ciencia & Vida, 2Universidad Andrés Bello, 3Universidad San Sebastián, 4University of California. [email protected] (228) M easurement of G L U T4 translocation and glucose uptake in skeletal muscle fibers from H F D-fed mice. 1 Y ildy Ut reras-M endoza, 1,2Alejandra Espinosa, 1Ariel Contreras, and 1Enrique Jaimovich. 1Laboratorio de Fisiología Celular del Músculo, Centro de Estudios Moleculares de la Célula, ICBM; 2 Escuela de Tecnología Médica, Facultad de Medicina, Universidad de Chile. [email protected] (230) Dendritic endoplasmic reticulum transport defines a non-canonical trafficking modality for G A B A B receptors. José V alenzuela 1 , Matías Jaureguiberry1,2, Viviana Valdés1, Omar Ramírez1, Thomas Blanpied3, and Andrés Couve1. 1Biomedical Neuroscience Institute, Facultad de Medicina, Universidad de Chile. 2School of Biochemistry, Faculty of Biological Science, Universidad Andrés Bello. 3Department of Physiology, University of Maryland School of Medicine, Baltimore, MD. [email protected] (232) Nitric O xide is a novel key factor in W nt-5a signaling in synapse. C arla Montecinos-Oliva 1, Rodrigo Varas2, Nibaldo C. Inestrosa1. 1Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2Departamento de Fisiología; Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. [email protected] (234) Runx2 transcription factor modulates expression of M M P2, M M P9 and M M P13, and enhance cellinvasion in human osteosa rcoma cells. K arina V illegas1,2, Sofía Jerez1,2, Oscar Vega1,2, Marcela Hernández3, Jorge Gamonal3, Gary S Stein4, Andre van Wijnen5 and Mario Galindo1,2. 1Millennium Institute on Immunology and Immunotherapy, 2Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile. 3Laboratorio de Biología Periodontal, Facultad de

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Odontología, Universidad de Chile. 4Department of Biochemistry, Vermont Cancer Center for Basic and Translational Research, University of Vermont. 5Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic. [email protected] (236) E valuating ionic conductances at the single vesicle level using an hybrid fret pair. Juan Pablo V ivar and Sebastián Brauchi. Instituto de Fisiología, Fac. de Medicina, Universidad Austral de Chile, Valdivia. [email protected] (238) Teneurin-4 is exp ressed in human ovarian ca rcinoma and is associated with tumor differentiation. 1 Ziegler A ., 1Graumann R., 1Vásquez M., 2Corvalán A., 3Casanello P., 4Brañes J.A., 5Roa I. 1Center for Human Genetics, Faculty of Medicine, Clínica Alemana-Universidad del Desarrollo; 2 Department of Hematology-Oncology, 3Perinatology Research Laboratory (PRL) & Cellular and Molecular Physiology Laboratory (CMPL), and 4Division of Obstetrics and Gynecology, School of Medicine, Pontificia Universidad Católica de Chile; 5Division of Pathology, Clínica Alemana de Santiago. [email protected] (Sponsor: C. Cabello). 19:00 – 20:30

Society M embers M eeting

20:30 – 21:30

Plena ry Lectu re Sociedad de Biologia C elula r de C hile Volcanes Room C hair: M aria Rosa Bono Ideas meta-metabólicas en el estudio de la dinámica celula r. Juan-C arlos Letelier, Departamento de Biología, Facultad de Ciencias, Universidad de Chile.

21:30

22:00

Awards C eremony Volcanes Room Nikon - Loncotec: Genexpress: Dinner and Party

Best Images in Cell Biology Best Presentations in O ral and Poster Communications